Document Detail


Cloning and expression of group IB phospholipase A2 isoforms in the red sea bream, Pagrus major.
MedLine Citation:
PMID:  11432463     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two cDNA encoding red sea bream DE-1 and DE-2 phospholipases A2 (PLA2) were cloned from the hepatopancreas of red sea bream, Pagrus (Chrysophrys) major. The cDNA of DE-1 PLA2 encoded a mature protein of 125 amino acid residues with an apparent signal peptide of 20 residues and propeptide of 5 residues, and that of DE-2 PLA2, a mature protein of 126 amino acid residues with an apparent signal peptide of 17 residues and propeptide of 6 residues. Comparison of the predicted amino acid sequences for mature DE-1 and DE-2 PLA2 showed that both proteins contain 14 cysteines including Cys 11 and 77 and a pancreatic loop, which are commonly conserved in group IB PLA2; however, the identity in amino acid sequence between DE-1 and DE-2 PLA2 was low (47%). A previous report concerning the cDNA cloning of red sea bream gill G-3 PLA2 and the present results represent the first cloning and sequencing of three distinct isoforms of group IB PLA2 in a single fish species, red sea bream. Reverse transcription-polymerase chain reaction analysis showed that DE-1 PLA2 mRNA was expressed in the hepatopancreas, pyloric ceca, intestine, spleen, gonad, stomach, and kidney, whereas gill G-3 PLA2 mRNA was expressed only in the gills and gonad. The expression of DE-2 PLA2 mRNA was detected in all of the tissues analyzed. These results indicate that three distinct isoforms of group IB PLA2, DE-1 and DE-2 PLA2 in hepatopanceas and gill G-3 PLA2, are expressed in a tissue-specific manner in red sea bream.
Authors:
N Iijima; Y Fujikawa; Y Tateishi; Y Takashima; S Uchiyama; M Esaka
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Lipids     Volume:  36     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-07-02     Completed Date:  2001-12-04     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  United States    
Other Details:
Languages:  eng     Pagination:  499-506     Citation Subset:  IM    
Affiliation:
Faculty of Applied Biological Science, Hiroshima University, Higashihiroshima, Japan. noriiij@hiroshima-u.ac.jp
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB009286;  AB050632;  AB050670
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
Isoenzymes / chemistry,  classification,  genetics,  metabolism
Molecular Sequence Data
Phospholipases A / chemistry,  classification*,  genetics*,  metabolism
Phospholipases A2
Phylogeny
RNA, Messenger / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sea Bream / genetics*
Sequence Alignment
Sequence Homology, Amino Acid
Chemical
Reg. No./Substance:
0/Isoenzymes; 0/RNA, Messenger; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2

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