| Cloning and expression of group IB phospholipase A2 isoforms in the red sea bream, Pagrus major. | |
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MedLine Citation:
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PMID: 11432463 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Two cDNA encoding red sea bream DE-1 and DE-2 phospholipases A2 (PLA2) were cloned from the hepatopancreas of red sea bream, Pagrus (Chrysophrys) major. The cDNA of DE-1 PLA2 encoded a mature protein of 125 amino acid residues with an apparent signal peptide of 20 residues and propeptide of 5 residues, and that of DE-2 PLA2, a mature protein of 126 amino acid residues with an apparent signal peptide of 17 residues and propeptide of 6 residues. Comparison of the predicted amino acid sequences for mature DE-1 and DE-2 PLA2 showed that both proteins contain 14 cysteines including Cys 11 and 77 and a pancreatic loop, which are commonly conserved in group IB PLA2; however, the identity in amino acid sequence between DE-1 and DE-2 PLA2 was low (47%). A previous report concerning the cDNA cloning of red sea bream gill G-3 PLA2 and the present results represent the first cloning and sequencing of three distinct isoforms of group IB PLA2 in a single fish species, red sea bream. Reverse transcription-polymerase chain reaction analysis showed that DE-1 PLA2 mRNA was expressed in the hepatopancreas, pyloric ceca, intestine, spleen, gonad, stomach, and kidney, whereas gill G-3 PLA2 mRNA was expressed only in the gills and gonad. The expression of DE-2 PLA2 mRNA was detected in all of the tissues analyzed. These results indicate that three distinct isoforms of group IB PLA2, DE-1 and DE-2 PLA2 in hepatopanceas and gill G-3 PLA2, are expressed in a tissue-specific manner in red sea bream. |
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Authors:
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N Iijima; Y Fujikawa; Y Tateishi; Y Takashima; S Uchiyama; M Esaka |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Lipids Volume: 36 ISSN: 0024-4201 ISO Abbreviation: Lipids Publication Date: 2001 May |
Date Detail:
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Created Date: 2001-07-02 Completed Date: 2001-12-04 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: United States |
Other Details:
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Languages: eng Pagination: 499-506 Citation Subset: IM |
Affiliation:
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Faculty of Applied Biological Science, Hiroshima University, Higashihiroshima, Japan. noriiij@hiroshima-u.ac.jp |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AB009286; AB050632; AB050670 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Cloning, Molecular Isoenzymes / chemistry, classification, genetics, metabolism Molecular Sequence Data Phospholipases A / chemistry, classification*, genetics*, metabolism Phospholipases A2 Phylogeny RNA, Messenger / genetics, metabolism Reverse Transcriptase Polymerase Chain Reaction Sea Bream / genetics* Sequence Alignment Sequence Homology, Amino Acid |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; 0/RNA, Messenger; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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