Document Detail


Cloning and expression of gene encoding a novel endoglycoceramidase of Rhodococcus sp. strain C9.
MedLine Citation:
PMID:  10876169     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Endoglycoceramidase (EGCase) is an enzyme capable of cleaving the glycosidic linkage between oligosaccharides and ceramides of various glycosphingolipids. We previously reported that the Asn-Glu-Pro (NEP) sequence is part of the active site of EGCase of Rhodococcus sp. strain M-777. This paper describes the molecular cloning of a new EGCase gene utilizing the NEP sequence from the genomic library of Rhodococcus sp. strain C9, which was clearly distinguishable from M-777 by 16S rDNA analysis. C9 EGCase possessed an open reading frame of 1,446 bp encoding 482 amino acids, and showed 78% and 76% identity to M-777 EGCase II at the nucleotide and amino acid levels, respectively. Interestingly, C9 EGCase showed the different specificity to the M-777 enzyme: it hydrolyzed b-series gangliotetraosylceramides more slowly than the M-777 enzyme, whereas both enzymes hydrolyzed a-series gangliosides and neutral glycosphingolipids to the same extent.
Authors:
K Sakaguchi; N Okino; N Sueyoshi; H Izu; M Ito
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biochemistry     Volume:  128     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  2000 Jul 
Date Detail:
Created Date:  2000-10-19     Completed Date:  2000-10-19     Revised Date:  2007-12-19    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  145-52     Citation Subset:  IM    
Affiliation:
Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, School Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Carbohydrate Sequence
Cloning, Molecular
Gangliosides / metabolism
Gene Expression Regulation, Enzymologic
Glycoside Hydrolases / genetics*,  metabolism*
Hydrogen-Ion Concentration
Kinetics
Molecular Sequence Data
Rhodococcus / enzymology*,  genetics
Sequence Analysis
Sequence Homology, Amino Acid
Substrate Specificity
Chemical
Reg. No./Substance:
0/Gangliosides; EC 3.2.1.-/Glycoside Hydrolases; EC 3.2.1.123/endoglycoceramidase

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