Document Detail


Cloning, expression, and characterization of a new beta-agarase from Vibrio sp. strain CN41.
MedLine Citation:
PMID:  21821738     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new agarase, AgaA(CN41), cloned from Vibrio sp. strain CN41, consists of 990 amino acids, with only 49% amino acid sequence identity with known β-agarases. AgaA(CN41) belongs to the GH50 (glycoside hydrolase 50) family but yields neoagarotetraose as the end product. AgaA(CN41) was expressed and characterized.
Authors:
Li Liao; Xue-Wei Xu; Xia-Wei Jiang; Yi Cao; Na Yi; Ying-Yi Huo; Yue-Hong Wu; Xu-Fen Zhu; Xin-Qi Zhang; Min Wu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-08-05
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  77     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-09-23     Completed Date:  2012-01-19     Revised Date:  2012-04-02    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7077-9     Citation Subset:  IM    
Affiliation:
College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/HM536934;  HM563685
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MeSH Terms
Descriptor/Qualifier:
Cloning, Molecular
Cluster Analysis
DNA, Bacterial / chemistry,  genetics
Gene Expression
Glycoside Hydrolases / biosynthesis*,  genetics*
Molecular Sequence Data
Phylogeny
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Vibrio / enzymology*,  genetics
Chemical
Reg. No./Substance:
0/DNA, Bacterial; EC 3.2.1.-/Glycoside Hydrolases; EC 3.2.1.81/agarase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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