Document Detail


Cloning and expression of caiDE genes in E. coli BL21(DE3)
MedLine Citation:
PMID:  12549051     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The caiDE genes for carnitine racemase and its relative factor were cloned into pUC18 from the chromosomal DNA of E. coli MC4100 by shot-gun approach and have been verified by sequence analysis. The target plasmid was named as pJX393 and subcloned to form a transcription plasmid pDSW2. After its transformation into BL21(DE3) strain and induction by IPTG, the recombinant strain containing pDSW2 expressed two apparent protein bands on SDS-PAGE, whose molecular weight were 30 kD and 24 kD individually.
Authors:
J Jiang; S Duan; H Zhang
Publication Detail:
Type:  English Abstract; Journal Article    
Journal Detail:
Title:  Wei sheng wu xue bao = Acta microbiologica Sinica     Volume:  40     ISSN:  0001-6209     ISO Abbreviation:  Wei Sheng Wu Xue Bao     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2003-01-28     Completed Date:  2004-08-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  21610860R     Medline TA:  Wei Sheng Wu Xue Bao     Country:  China    
Other Details:
Languages:  chi     Pagination:  586-90     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Bio-Reactorand Engineering, ECUST, Shanghai 200237.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Cloning, Molecular
DNA, Bacterial / genetics
Escherichia coli / genetics*
Escherichia coli Proteins / genetics*
Gene Expression
Molecular Sequence Data
Plasmids
Racemases and Epimerases / genetics*
Recombinant Proteins / biosynthesis*
Transformation, Bacterial
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/Escherichia coli Proteins; 0/Recombinant Proteins; EC 5.1.-/Racemases and Epimerases; EC 5.1.-/caiD protein, E coli

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