| Cloning and expression of cDNA encoding human placental estrogen sulfotransferase. | |
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MedLine Citation:
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PMID: 8187949 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Using two oligoprimers derived from the bovine placental estrogen sulfotransferase sequence, we amplified a probe for human placental estrogen sulfotransferase. Using this probe to screen a human placental cDNA library constructed in lambda gt11, we isolated a cDNA clone of 1.3 kb encoding human estrogen sulfotransferase. DNA analysis predicts a protein of 295 amino acids with a calculated molecular weight of 34,199. Alignment of the amino acid sequence with other sulfotransferases indicates that human placental estrogen sulfotransferase shares 68.6, 68.2 and 65.9% similarity with bovine placental, guinea pig adrenocortical, and rat liver estrogen sulfotransferase, respectively. It shows also 95.6, 57.6, 85.3, and 54.2% similarity to human phenol, human DHEA, rat phenol, and rat hydroxysteroid sulfotransferase, respectively. Transfection of expression vectors encoding human estrogen sulfotransferase and dehydroepiandrosterone (DHEA) sulfotransferase in human adrenal adenocarcinoma SW-13 cells indicates that estrogen sulfotransferase transforms estrone more specifically, whereas DHEA sulfotransferase is more specific for DHEA and pregnenolone. |
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Authors:
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F Bernier; I Lopez Solache; F Labrie; V Luu-The |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular and cellular endocrinology Volume: 99 ISSN: 0303-7207 ISO Abbreviation: Mol. Cell. Endocrinol. Publication Date: 1994 Feb |
Date Detail:
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Created Date: 1994-06-23 Completed Date: 1994-06-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7500844 Medline TA: Mol Cell Endocrinol Country: IRELAND |
Other Details:
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Languages: eng Pagination: R11-5 Citation Subset: IM |
Affiliation:
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Medical Research Council Group in Molecular Endocrinology, CHUL Research Center, Quebec, Canada. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/L25275 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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pathology Animals Base Sequence Cattle Cloning, Molecular DNA, Complementary / genetics Female Guinea Pigs Humans Molecular Sequence Data Pregnancy Proteins / biosynthesis, genetics* Rats Recombinant Fusion Proteins / biosynthesis Sequence Alignment Sequence Homology, Amino Acid Species Specificity Sulfotransferases / biosynthesis, genetics* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/Pregnancy Proteins; 0/Recombinant Fusion Proteins; EC 2.8.2.-/Sulfotransferases; EC 2.8.2.4/estrone sulfotransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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