Document Detail


Cloning, expression, and biological activity of growth hormone in bullfrog (Rana catesbeiana).
MedLine Citation:
PMID:  16442533     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cDNA (GenBank, AY251538) encoding bullfrog growth hormone (fGH) was cloned by RT-PCR from the total RNA of pituitary glands. Its sequence encoded a putative polypeptide of 215 amino acids, including a signal peptide of 25 amino acids with no change to those of other previous reported bullfrog GHs. The fGH precursor shares 98.1, 96.3, and 95.3% homologies to those of other bullfrog GHs (AAB24792, AAB19428, CAA31038) in amino-acid sequence and its nucleotide sequences of the coding region shares 99.1 and 98.5% homologies to those of previous bullfrog GH genes (S52027 and X12520). The fGH cDNA was also efficiently expressed in Escherichia coli carrying a plasmid pGfGH in which the cDNA was under the control of GST promoter of pGEX1-lambdaT. The expressed fusion protein GST-fGH is comprised about 29.3% of the total cellular protein in such bacteria. The purified GST-fGH cannot only showed a obvious dose-response curve when it reacted with the hepatic membrane receptor proteins from bullfrog, but also significantly increased the body weight and length of bullfrog after twice injection and such effects lasted two or three weeks after the last injection with purified GST-fGH.
Authors:
Lin Yu; Xiaomei Chen; Shigui Liu; Zhangfu Long
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-01-26
Journal Detail:
Title:  General and comparative endocrinology     Volume:  146     ISSN:  0016-6480     ISO Abbreviation:  Gen. Comp. Endocrinol.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-04-24     Completed Date:  2006-06-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370735     Medline TA:  Gen Comp Endocrinol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  296-303     Citation Subset:  IM    
Affiliation:
Key Laboratory of Bio-Resource and Eco-environment, Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, PR China.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AAP04356;  AY251538
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Cloning, Molecular
Glutathione Transferase / genetics
Growth Hormone / genetics*,  pharmacology
Molecular Sequence Data
Rana catesbeiana* / growth & development
Recombinant Fusion Proteins / metabolism
Sequence Alignment
Chemical
Reg. No./Substance:
0/Recombinant Fusion Proteins; 9002-72-6/Growth Hormone; EC 2.5.1.18/Glutathione Transferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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