Document Detail

Cloning and expression analysis of goldfish (Carassius auratus L.) prominin.
MedLine Citation:
PMID:  17123832     Owner:  NLM     Status:  MEDLINE    
The integral membrane protein known as prominin was first identified on the apical surface of mouse neural epithelial cells as well as on the surface of human haematopoietic progenitor cells. This report describes a prominin-like sequence and expression analysis of the prominin in the goldfish. The predicted amino acid sequence for goldfish prominin shares all of the hallmark structural characteristics of the prominin family, however the relatedness assessed using the percent amino acid identity indicated that goldfish prominin cannot be placed into the current mammalian dichotomy of type 1 or 2. The real time PCR analyses indicated that prominin was broadly expressed in different tissues with particularly high levels observed in the kidney and gill of the goldfish. Goldfish prominin was also found to be differentially expressed in subpopulations of in vitro-derived goldfish macrophages, with the highest expression observed in progenitor cells.
John G Walsh; Daniel R Barreda; Miodrag Belosevic
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-04-28
Journal Detail:
Title:  Fish & shellfish immunology     Volume:  22     ISSN:  1050-4648     ISO Abbreviation:  Fish Shellfish Immunol.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2006-12-25     Completed Date:  2007-05-10     Revised Date:  2010-07-16    
Medline Journal Info:
Nlm Unique ID:  9505220     Medline TA:  Fish Shellfish Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  308-17     Citation Subset:  IM    
Department of Biological Sciences, CW-405 Biological Sciences Centre, University of Alberta, Edmonton, Alta T6G 2E9, Canada.
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MeSH Terms
Actins / analysis,  biosynthesis
Amino Acid Sequence
Antigens, CD / biosynthesis*,  chemistry,  genetics*
Base Sequence
Blotting, Southern / veterinary
Cloning, Molecular*
DNA Primers / chemistry
DNA, Complementary / chemistry
Gene Expression Profiling / veterinary
Glycoproteins / biosynthesis*,  chemistry,  genetics*
Goldfish / genetics*,  immunology,  metabolism
Macrophages / physiology
Molecular Sequence Data
Peptides / chemistry,  genetics*
RNA, Messenger / analysis,  biosynthesis
Reverse Transcriptase Polymerase Chain Reaction / veterinary
Sequence Homology, Amino Acid
Reg. No./Substance:
0/AC133 antigen; 0/Actins; 0/Antigens, CD; 0/DNA Primers; 0/DNA, Complementary; 0/Glycoproteins; 0/Peptides; 0/RNA, Messenger
Erratum In:
Fish Shellfish Immunol. 2010 Sep;29(3):550

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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