| Cloning and differentiation-induced expression of a murine serotonin1A receptor in a septal cell line. | |
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MedLine Citation:
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PMID: 8254366 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A neuronal cell model endogenously expressing the 5-HT1A receptor, in which to study the function and regulation of this gene, has yet to be identified. We examined murine SN-48 cells, a septum x neuroblastoma fusion cell line that proliferates in a nondifferentiated state but can be induced to differentiate into neurofilament-positive cells following 24-96 hr treatment with 10 microM retinoic acid in low serum. Northern blot analysis demonstrated the presence of a single 10.9 kilobase (kb) 5-HT1A receptor RNA species in differentiated SN-48 cells, which was not detected in undifferentiated SN-48 cells. The presence of receptor RNA in differentiated SN-48 cells correlated with the appearance of functional responses (i.e., pertussis toxin-sensitive inhibition of cAMP accumulation) to 5-HT1A agonists in differentiated but not in undifferentiated cells. In order to verify that the large 10.9 kb RNA species in SN-48 cells truly corresponded to the mouse 5-HT1A receptor RNA, a cDNA fragment from differentiated SN-48 cells was used to clone the corresponding mouse brain cDNA. The 2.4 kb cDNA contained a single open reading frame that displayed high (> 85% predicted amino acid identity) homology to the human and rat 5-HT1A receptor genes. When transfected into receptor-negative Ltk- cells, this cDNA was found to direct expression of a murine 5-HT1A receptor. Thus, we conclude that upon differentiation SN-48 cells express RNA encoding functional 5-HT1A receptors. The SN-48 septal cells will provide a useful cellular model system for investigating aspects of neuronal differentiation leading to the development of sensitivity to serotonergic input. |
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Authors:
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A Charest; B H Wainer; P R Albert |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of neuroscience : the official journal of the Society for Neuroscience Volume: 13 ISSN: 0270-6474 ISO Abbreviation: J. Neurosci. Publication Date: 1993 Dec |
Date Detail:
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Created Date: 1994-01-12 Completed Date: 1994-01-12 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8102140 Medline TA: J Neurosci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 5164-71 Citation Subset: IM |
Affiliation:
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Department of Pharmacology and Therapeutics, McGill University, Montreal, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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8-Hydroxy-2-(di-n-propylamino)tetralin
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pharmacology Animals Base Sequence Cell Differentiation / drug effects Cloning, Molecular* Gene Expression* Hippocampus / cytology, metabolism Mice Molecular Sequence Data Neuroblastoma / metabolism Neurons / cytology, metabolism RNA, Messenger / genetics, metabolism Receptors, Serotonin / genetics*, metabolism* Septal Nuclei / cytology, metabolism* Tretinoin / pharmacology Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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NS-25787/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/Receptors, Serotonin; 302-79-4/Tretinoin; 78950-78-4/8-Hydroxy-2-(di-n-propylamino)tetralin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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