Document Detail

Cloning and complete nucleotide sequences of the type II restriction-modification genes of Salmonella infantis.
MedLine Citation:
PMID:  2836359     Owner:  NLM     Status:  MEDLINE    
The complete type II restriction-modification system of Salmonella infantis was cloned in Escherichia coli as an R . Sau3AI fragment of 3,430 base pairs. The clone was shown to express the restriction endonuclease as well as the modification methylase. The nucleotide sequence of the above fragment showed two open reading frames of 461 and 230 codons in tail-to-tail orientation. These were shown to represent the modification methylase M . SinI and the restriction endonuclease R . SinI, respectively. The methylase M . SinI amino acid sequence revealed a considerable similarity to those of other deoxycytidylate methylases. In contrast, endonuclease R . SinI did not exhibit such a similarity to other restriction enzymes.
C Karreman; A de Waard
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of bacteriology     Volume:  170     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1988 Jun 
Date Detail:
Created Date:  1988-07-13     Completed Date:  1988-07-13     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2527-32     Citation Subset:  IM    
Department of Medical Biochemistry, Sylvius Laboratories, University of Leiden, The Netherlands.
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MeSH Terms
Amino Acids / analysis
Base Sequence
Cloning, Molecular*
DNA Restriction Enzymes / metabolism
DNA-Cytosine Methylases*
Deoxyribonucleases, Type II Site-Specific*
Methyltransferases / metabolism
Molecular Sequence Data
Salmonella / genetics*
Transcription, Genetic
Reg. No./Substance:
0/Amino Acids; 0/Codon; EC 2.1.1.-/DNA modification methylase M.SinI; EC 2.1.1.-/DNA-Cytosine Methylases; EC 2.1.1.-/Methyltransferases; EC 3.1.21.-/DNA Restriction Enzymes; EC, Type II Site-Specific; EC type II deoxyribonucleases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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