Document Detail

Cloning and complete nucleotide sequences of the type II restriction-modification genes of Salmonella infantis.
MedLine Citation:
PMID:  2836359     Owner:  NLM     Status:  MEDLINE    
The complete type II restriction-modification system of Salmonella infantis was cloned in Escherichia coli as an R . Sau3AI fragment of 3,430 base pairs. The clone was shown to express the restriction endonuclease as well as the modification methylase. The nucleotide sequence of the above fragment showed two open reading frames of 461 and 230 codons in tail-to-tail orientation. These were shown to represent the modification methylase M . SinI and the restriction endonuclease R . SinI, respectively. The methylase M . SinI amino acid sequence revealed a considerable similarity to those of other deoxycytidylate methylases. In contrast, endonuclease R . SinI did not exhibit such a similarity to other restriction enzymes.
C Karreman; A de Waard
Related Documents :
12719939 - Cloning and characterization of the gene coding for the aerobic azoreductase from pigme...
2044939 - Cloning and sequencing of the fus-gene encoding elongation factor 2 in the archaebacter...
2829719 - Genetic and physical characterization of proba genes of the marine bacterium vibrio par...
9116039 - Cloning and expression of the rubredoxin gene from desulfovibrio vulgaris (miyazaki f)-...
1885539 - Cloning, nucleotide sequence, and expression of the pasteurella haemolytica a1 glycopro...
6235149 - Nucleotide sequence of gene pfkb encoding the minor phosphofructokinase of escherichia ...
9511749 - Determination of coxiella burnetii rpob sequence and its use for phylogenetic analysis.
11532219 - The genome of mycobacterium leprae: a minimal mycobacterial gene set.
24637409 - The genomic rna1 and rna2 sequences of the tobacco rattle virus isolates found in polis...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of bacteriology     Volume:  170     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1988 Jun 
Date Detail:
Created Date:  1988-07-13     Completed Date:  1988-07-13     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2527-32     Citation Subset:  IM    
Department of Medical Biochemistry, Sylvius Laboratories, University of Leiden, The Netherlands.
Data Bank Information
Bank Name/Acc. No.:
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acids / analysis
Base Sequence
Cloning, Molecular*
DNA Restriction Enzymes / metabolism
DNA-Cytosine Methylases*
Deoxyribonucleases, Type II Site-Specific*
Methyltransferases / metabolism
Molecular Sequence Data
Salmonella / genetics*
Transcription, Genetic
Reg. No./Substance:
0/Amino Acids; 0/Codon; EC 2.1.1.-/DNA modification methylase M.SinI; EC 2.1.1.-/DNA-Cytosine Methylases; EC 2.1.1.-/Methyltransferases; EC 3.1.21.-/DNA Restriction Enzymes; EC, Type II Site-Specific; EC type II deoxyribonucleases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Overproduction of single-stranded-DNA-binding protein specifically inhibits recombination of UV-irra...
Next Document:  Isolation and characterization of the modification methylase M . SinI.