Document Detail

Cloning and characterization of rice (Oryza sativa L) telomerase reverse transcriptase, which reveals complex splicing patterns.
MedLine Citation:
PMID:  12100484     Owner:  NLM     Status:  MEDLINE    
Plant chromosomes terminate in telomeres as in other eukaryotes. Telomeres are vital to genome stability and their malfunctioning is lethal. One of the core components of the telomere complex is telomerase. The enzyme activity depends on RNA (TER) and reverse transcriptase (TERT) subunits. We describe here the isolation, sequencing and characterization of the telomerase reverse transcriptase catalytic subunit from the monocot plant Oryza sativa L. (OsTERT). A single copy of this gene is present in the rice genome. The protein predicted from the OsTERT sequence has all the signature motifs of the TERT family members. Our data indicate that rice telomerase activity is developmentally regulated and is high in in vitro tissue and cell culture. However, steady-state transcript levels of the TERT gene do not seem to correlate with enzyme activity. Northern and RT-PCR analyses of the OsTERT gene transcript profile show multiple differentially spliced transcripts in both telomerase-positive and telomerase-negative tissues. Based on quantitative analysis of these transcripts, we speculate that the overall balance between the quantities of particular alternatively spliced transcripts may determine whether the TERT protein(s) is active or not. The diversity of splicing variants detected suggests that, as recently discovered for mammalian TERT proteins, rice TERT protein variants may perform functions other than telomere maintenance.
Katarzyna Heller-Uszynska; Wendelin Schnippenkoetter; Andrzej Kilian
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  31     ISSN:  0960-7412     ISO Abbreviation:  Plant J.     Publication Date:  2002 Jul 
Date Detail:
Created Date:  2002-07-08     Completed Date:  2002-09-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  England    
Other Details:
Languages:  eng     Pagination:  75-86     Citation Subset:  IM    
CAMBIA: Center for the Application of Molecular Biology to International Agriculture, GPO Box 3200, Canberra, ACT 2601, Australia.
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MeSH Terms
Alternative Splicing
Amino Acid Sequence
Arabidopsis / enzymology,  genetics
Arabidopsis Proteins / genetics
Base Sequence
Cloning, Molecular
DNA, Plant / genetics
DNA-Binding Proteins
Genes, Plant
Molecular Sequence Data
Oryza sativa / enzymology*,  genetics*
Plant Proteins / chemistry,  genetics
Sequence Homology, Amino Acid
Telomerase / chemistry,  genetics*
Reg. No./Substance:
0/Arabidopsis Proteins; 0/DNA, Plant; 0/DNA-Binding Proteins; 0/Plant Proteins; EC

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