Document Detail


Cloning and characterization of the ribosomal protein genes in the spc operon of a prokaryotic endosymbiont of the pea aphid, Acyrthosiphon kondoi.
MedLine Citation:
PMID:  7584036     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To correlate a prokaryotic endosymbiont in the pea aphid, Acyrthosiphon kondoi, with the endosymbionts in related aphid species as well as with free-living bacteria and subcellular organelles, and to study the mode of its gene expression within aphid cells, we have cloned and characterized the genes encoding ribosomal proteins S3, L16, L29, S17, L14, L24, L5, S14, S8, L6, L18, S5, L30, L15 and secretion protein Y (Sec Y) from the S10 and spc ribosomal protein gene operons of this endosymbiont. The organization of these genes is identical to that in Escherichia coli, and their nucleotide sequences are highly similar (87% identity) to the corresponding E. coli genes. They are much less similar to the corresponding chloroplast and mitochondrial genes. The guanine plus cytosine G+C content of the genes of the A. kondoi endosymbiont is much higher than those of the endosymbionts in related aphid species reported so far. It appears either that the A. kondoi endosymbiont is derived from an ancestral bacterium different from those in other aphids or that its G+C content increased in a relatively short time after the evolutionary divergence of its host.
Authors:
R Abe; A Yamashita; K Isono
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  DNA research : an international journal for rapid publication of reports on genes and genomes     Volume:  1     ISSN:  1340-2838     ISO Abbreviation:  DNA Res.     Publication Date:  1994  
Date Detail:
Created Date:  1995-12-06     Completed Date:  1995-12-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9423827     Medline TA:  DNA Res     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  103-14     Citation Subset:  IM    
Affiliation:
Postgraduate School of Science and Technology, Kobe University, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/D31786
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Aphids / microbiology*
Bacteria / genetics*
Bacterial Proteins / genetics*
Base Composition
Base Sequence
Cloning, Molecular
Escherichia coli / genetics
Escherichia coli Proteins*
Genes, Bacterial / genetics*
Molecular Sequence Data
Operon / genetics
Peas / parasitology
Ribosomal Proteins / genetics*
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Symbiosis
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Escherichia coli Proteins; 0/Ribosomal Proteins; 0/SecY protein, E coli

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