| Cloning and characterization of polyketide synthase genes for jadomycin B biosynthesis in Streptomyces venezuelae ISP5230. | |
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MedLine Citation:
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PMID: 7881555 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hybridizing fragments in the genomic DNA of Streptomyces venezuelae ISP5230, which produces the jadomycin group of angucycline antibiotics, were detected by probing with actI DNA from Streptomyces coelicolor A3(2). The hybridizing regions were isolated from a 16.5 kb insert of S. venezuelae DNA recovered from a genomic library cloned in a lambda replacement vector. Subcloning and sequencing of a 4.8 kb segment of the insert, containing regions hybridizing to actIII as well as actI, identified five open reading frames (ORFs). The deduced polypeptide products of the ORFs closely resemble in sequence the components of streptomycete type-II polyketide synthases (PKSs): the ORF1 product corresponds to the ketoacyl synthase, and the ORF2 product to a polypeptide closely related to the ketoacyl synthase and involved in determining chain length; the ORF3 product matches the acyl carrier protein; ORF4 encodes a bifunctional cyclase/dehydrase; and ORF5 encodes a ketoreductase. Integration into the chromosomal DNA of a plasmid containing a segment of the ORF2-ORF4 region severely depressed jadomycin B biosynthesis; since the integrant showed no change in growth or spore pigmentation, the cloned PKS genes are presumed to encode enzymes in the pathway for jadomycin biosynthesis. |
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Authors:
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L Han; K Yang; E Ramalingam; R H Mosher; L C Vining |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Microbiology (Reading, England) Volume: 140 ( Pt 12) ISSN: 1350-0872 ISO Abbreviation: Microbiology (Reading, Engl.) Publication Date: 1994 Dec |
Date Detail:
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Created Date: 1995-04-07 Completed Date: 1995-04-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9430468 Medline TA: Microbiology Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 3379-89 Citation Subset: IM |
Affiliation:
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Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/L33245 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Anti-Bacterial Agents / biosynthesis* Base Sequence Cloning, Molecular DNA, Bacterial / genetics Genes, Bacterial* Isoquinolines / metabolism Molecular Sequence Data Multienzyme Complexes / genetics* Multigene Family Nucleic Acid Hybridization Open Reading Frames Restriction Mapping Sequence Homology, Amino Acid Streptomyces / enzymology, genetics*, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/DNA, Bacterial; 0/Isoquinolines; 0/Multienzyme Complexes; 149633-99-8/jadomycin B |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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