| Cloning and characterization of a novel gene which encodes a protein interacting with the mitosis-associated kinase-like protein NTKL. | |
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MedLine Citation:
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PMID: 12783284 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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NTKL is an evolutionarily conserved kinase-like protein. The cell-cycle-dependent centrosomal localization of NTKL suggested that it was involved in centrosome-related cellular function. The mouse NTKL protein is highly homologous with human NTKL. A novel mouse protein was identified as an NTKL-binding protein (NTKL-BP1) by yeast two-hybrid screening, and the full-length cDNA was amplified based on the result of a sequence data analysis cloning strategy. The full-length cDNA sequence of the NTKL-BP1 gene consists of 2,537 bp, which encode 368 amino acids. A database search revealed that homologues of NTKL-BP1 exist in different organisms, including Arabidopsis thaliana, Drosophila melanogaster, Plasmodium falciparum, Geobacter metallireducens, Anopheles gambiae and human. It suggests that NTKL-BP1 is an evolutionarily conserved protein. The expression of NTKL-BP1 was observed in multiple normal mouse tissues. The interaction of the two proteins was confirmed by co-immunoprecipitation. Moreover, immunofluorescent staining indicated that NTKL and NTKL-BP1 were all localized in the cytoplasm. |
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Authors:
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Yujun Di; Jinjun Li; Ji Fang; Zhigang Xu; Xianghuo He; Fengrui Zhang; Jiqiang Ling; Xinrui Li; Dongbin Xu; Li Li; Yu-Yang Li; Keke Huo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2003-05-29 |
Journal Detail:
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Title: Journal of human genetics Volume: 48 ISSN: 1434-5161 ISO Abbreviation: J. Hum. Genet. Publication Date: 2003 |
Date Detail:
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Created Date: 2003-07-01 Completed Date: 2004-02-24 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9808008 Medline TA: J Hum Genet Country: Japan |
Other Details:
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Languages: eng Pagination: 315-21 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 200433 Shanghai, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Carrier Proteins / chemistry, genetics* Cell Line, Tumor Cloning, Molecular Cytoplasm / metabolism Databases, Genetic Fluorescent Antibody Technique, Direct Mice Mitosis Molecular Sequence Data Precipitin Tests Protein Kinases / chemistry*, genetics* Protein Structure, Tertiary Sequence Homology, Amino Acid Tissue Distribution Two-Hybrid System Techniques |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; EC 2.7.-/Protein Kinases; EC 2.7.1.-/NTKL-BP1 protein, mouse |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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