Document Detail


Cloning and characterization of a novel CoA-ligase gene from Penicillium chrysogenum.
MedLine Citation:
PMID:  21625874     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A novel phenylacetic acid (PAA)-induced CoA-ligase-encoding gene, designated as phlC, has been cloned from penicillin-producing fungus Penicillium chrysogenum. The open reading frame of phlC cDNA was 1671 bp and encoded a 556 amino acid residues protein with the consensus AMP binding site and a peroxisomal targeting signal 1 on its C terminus. The deduced amino acid sequence showed 37% and 38% identity with characterized P. chrysogenum Phl and PhlB protein, respectively. Functional recombinant PhlC protein was overexpressed in Escherichia coli. The purified recombinant enzyme was capable to convert PAA into its corresponding CoA ester with a specific activity of 129.5 ± 3.026 pmol/min per mg protein. Similar to Phl and PhlB, PhlC displayed broad substrate spectrum and showed higher activities to medium- and long-chain fatty acids. The catalytic properties of PhlC have been determined and compared to those of Phl and PhlB.
Authors:
Zhou-Liang Yu; Jing Liu; Fu-Qiang Wang; Meng Dai; Bao-Hua Zhao; Jian-Gong He; Hua Zhang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-31
Journal Detail:
Title:  Folia microbiologica     Volume:  -     ISSN:  1874-9356     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-5-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376757     Medline TA:  Folia Microbiol (Praha)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Bioresources, New Drug R&D Center of North China Pharmaceutical Corporation and National Engineering Research Center for Microbial Medicine, 388 East Heping Road, Shijiazhuang, 050015, China.
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