Document Detail


Cloning and characterization of liver progenitor cells from the scattered cell clusters in primary culture of porcine livers.
MedLine Citation:
PMID:  18468248     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The scattered cell clusters that can differentiate into hepatocytes or biliary epithelial cells have been isolated from primary cultures of adult porcine livers. We have generated 11 clonal cell lines from this system and identified liver progenitor cells (LPCs) among the clonal lines. These clonal lines expressed c-kit, HNF-1, HNF-6, and/or CK19 mRNA. An immunocytochemical study of the clonal lines indicated that clonal line CL-11 expressed liver epithelial cell markers CK14, vimentin, CK18, and BD-1. The expression of albumin and alpha1-antitrypsin (alpha1-AT) mRNA was only upregulated in CL-11 among the clonal lines when they were grown as aggregates. Under these conditions, CL-11 also exhibited ammonia metabolic activity and several indicators that suggest hepatocytic differentiation, including the upregulation of liver-specific genes such as dipeptidyl peptidase IV, CYP1A1, and CYP3A4 mRNA, and the downregulation of biliary cell markers such as gamma-glutamyltrans-peptidase (GGT), CK19, and HNF6 mRNA. After culturing CL-11 in Matrigel, the expression of GGT and HNF6 mRNA was upregulated. These results indicate that CL-11 has dual potential: the ability to differentiate as hepatocytes or as bile duct cells. The isolation of scattered cells could provide a simple method to generate LPC lines from adult livers.
Authors:
Takayoshi Tokiwa; Taisuke Yamazaki; Masashi Ono; Shin Enosawa; Takashi Tsukiyama
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell transplantation     Volume:  17     ISSN:  0963-6897     ISO Abbreviation:  Cell Transplant     Publication Date:  2008  
Date Detail:
Created Date:  2008-05-12     Completed Date:  2008-10-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9208854     Medline TA:  Cell Transplant     Country:  United States    
Other Details:
Languages:  eng     Pagination:  179-86     Citation Subset:  IM    
Affiliation:
Department of Liver Cell Biology, Kohno Clinical Medicine Research Institute, Tokyo 140-0001, Japan. t.tokiwa@kcmi.or.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD26 / biosynthesis
Bile Ducts / cytology
Biological Markers / analysis
Cell Culture Techniques
Cell Differentiation
Clone Cells
Cytochrome P-450 CYP3A / biosynthesis
Cytochrome P-450 Enzyme System / biosynthesis
Epithelial Cells / cytology,  physiology
Hepatocyte Nuclear Factor 4 / biosynthesis
Hepatocyte Nuclear Factor 6 / biosynthesis
Hepatocytes* / cytology,  physiology
Stem Cells* / cytology,  physiology
Sus scrofa
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Hepatocyte Nuclear Factor 4; 0/Hepatocyte Nuclear Factor 6; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.14.13.67/CYP3A4 protein, human; EC 1.14.14.1/Cytochrome P-450 CYP3A; EC 3.4.14.5/Antigens, CD26; EC 3.4.14.5/DPP4 protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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