Document Detail


Cloning and characterization of the kinesin-related protein, Krp1p, in Schizosaccharomyces pombe.
MedLine Citation:
PMID:  12132578     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Kinesin have been cloned in many organisms. They played important roles in the transport of cell organelles, polarized growth, and secretion. We report here the identification of a kinesin-related protein in Schizosaccharomyces pombe, which was named kinesin-related protein (Krplp). The primer sequences were driven from the highly conserved area of the kinesin genes in other organisms. We cloned kinesin genes from S. pombe using the PCR technique. Sequence analysis revealed that krp1+ has a 1,665 bp open-reading frame (ORF) that encoded a protein that consisted of 554 amino acids with a molecular weight of 61,900. It is homologous to the proteins that belong to the kinesin heavy chain (KHC) superfamily [GenBank accession No. AF156966 (genomic DNA) and AF247188 (mRNA)]. To characterize Krplp, the gene was disrupted and overexpressed in S. pombe. Cells that contained a krp1+ null allele were viable. Overexpression of Krp1p resulted in the inhibition of mitotic growth; cells became elongated, branched, and formed aberrant septa. To identify proteins that interact with Krplp, the yeast two-hybrid system was used. As a result, the novel protein, designated kinesin associated protein (Kap1p), was identified and showed structural homology to the proteins of the myosin family (GenBank accession No. AF351206). The data from the overexpression and two-hybrid study of Krplp may provide information that Krplp can have roles in cytokinesis with myosin.
Authors:
Jae Wook Jeong; Dong Keun Rhee; Soo Young Cho; Kwang Lae Hae; Dong Uk Kim; Misun Won; Hyong Bai Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecules and cells     Volume:  13     ISSN:  1016-8478     ISO Abbreviation:  Mol. Cells     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-07-22     Completed Date:  2003-08-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9610936     Medline TA:  Mol Cells     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  389-98     Citation Subset:  IM    
Affiliation:
Division of Molecular Biology, Graduate School of Biotechnology, Korea University, Seoul.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF156966;  AF247188;  AF351206
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Cell Division / drug effects
Cell Size / drug effects
Cloning, Molecular
Gene Deletion
Kinesin / genetics*,  isolation & purification,  pharmacology
Molecular Sequence Data
Mutagenesis
Protein Binding
Schizosaccharomyces / chemistry,  genetics*
Schizosaccharomyces pombe Proteins / genetics*,  isolation & purification,  pharmacology
Sequence Analysis, DNA
Transduction, Genetic
Two-Hybrid System Techniques
Chemical
Reg. No./Substance:
0/Schizosaccharomyces pombe Proteins; EC 3.6.1.-/Kinesin; EC 3.6.1.-/Krp1 protein, S pombe

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