Document Detail


Cloning and characterization of a β-ketoacyl-acyl carrier protein synthase II from Jatropha curcas.
MedLine Citation:
PMID:  22424763     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A cDNA clone encoding a putative β-ketoacyl-acyl carrier protein (ACP) synthase II (KASII), a key enzyme in fatty acid biosynthesis, was isolated from Jatropha curcas L., a woody oil plant. The isolated cDNA clone of JcKASII contained a 1722-bp open reading frame coding for 573 amino acids with a predicted molecular mass of about 60.98kDa and the conserved Cys(324) residues that has been proposed as the active site of KASII proteins. The deduced amino acid sequence of the cDNA clone had about 70-84% identity with the KASII from other plants. The transcript of JcKASII was detected in all tissues examined and increased during seed maturation. Expression of JcKASII in the Arabidopsis KASII mutant (fab1) could complement the fatty acid composition of the mutant. Overexpression of JcKASII cDNA under the cauliflower mosaic virus 35S promoter in Arabidopsis resulted in decreasing 16-carbon fatty acids and increasing 18-carbon fatty acids in leaves and seeds. Taken together, these results show that JcKASII could function in 18-carbon fatty acids accumulation in plant and may be useful in the genetic engineering of J. curcas.
Authors:
Qian Wei; Jun Li; Lin Zhang; Pingzhi Wu; Yaping Chen; Meiru Li; Huawu Jiang; Guojiang Wu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-16
Journal Detail:
Title:  Journal of plant physiology     Volume:  -     ISSN:  1618-1328     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882059     Medline TA:  J Plant Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Crown Copyright © 2012. Published by Elsevier GmbH. All rights reserved.
Affiliation:
Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, PR China; Graduate University of the Chinese Academy of Sciences, Beijing 100049, PR China.
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