Document Detail


Cloning and characterization of an inhibitor of apoptosis protein (IAP) from Bombyx mori.
MedLine Citation:
PMID:  11341966     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We cloned a novel inhibitor of apoptosis protein (IAP) family member, BmIAP, from Bombyx mori BmN cells. BmIAP contains two baculoviral IAP repeat (BIR) domains followed by a RING domain. BmIAP shares striking amino acid sequence similarity with lepidopteran IAPs, SfIAP and TnIAP, and with two baculoviral IAPs, CpIAP and OpIAP, suggesting evolutionary conservation. BmIAP blocks programmed cell death (apoptosis) in Spodoptera frugiperda Sf-21 cells induced by p35 deficient Autographa californica nucleopolyhedrovirus (AcMNPV). This anti-apoptotic function requires both the BIR domains and RING domain of BmIAP. In mammalian cells, BmIAP inhibits Bax induced but not Fas induced apoptosis. Further biochemical data suggest that BmIAP is a specific inhibitor of mammalian caspase-9, an initiator caspase in the mitochondria/cytochrome-c pathway, but not the downstream effector proteases, caspase-3 and caspase-7. These results suggest that suppression of apoptosis by lepidopteran IAPs in insect cells may involve inhibition of an upstream initiator caspase in the conserved mitochondria/cytochrome-c pathway for apoptosis.
Authors:
Q Huang; Q L Deveraux; S Maeda; H R Stennicke; B D Hammock; J C Reed
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1499     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-05-08     Completed Date:  2001-05-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  191-8     Citation Subset:  IM    
Affiliation:
Department of Entomology, University of California, Davis 95616, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Apoptosis
Baculoviridae / genetics
Base Sequence
Bombyx / genetics*
Caspase 9
Caspases / antagonists & inhibitors,  metabolism
Cell Line
Cloning, Molecular
Cysteine Proteinase Inhibitors / metabolism
DNA Primers / genetics
Drosophila Proteins*
Inhibitor of Apoptosis Proteins
Insect Proteins / chemistry,  genetics*
Molecular Sequence Data
Protein Structure, Tertiary
Proto-Oncogene Proteins / metabolism
Proto-Oncogene Proteins c-bcl-2*
Spodoptera
bcl-2-Associated X Protein
Grant Support
ID/Acronym/Agency:
AG15402/AG/NIA NIH HHS; CA30199/CA/NCI NIH HHS; ES 05707/ES/NIEHS NIH HHS; ES02701/ES/NIEHS NIH HHS; ES04699/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Cysteine Proteinase Inhibitors; 0/DIAP2 protein, Drosophila; 0/DNA Primers; 0/Drosophila Proteins; 0/Inhibitor of Apoptosis Proteins; 0/Insect Proteins; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; EC 3.4.22.-/Caspase 9; EC 3.4.22.-/Caspases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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