| Cloning and characterization of an inhibitor of apoptosis protein (IAP) from Bombyx mori. | |
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MedLine Citation:
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PMID: 11341966 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We cloned a novel inhibitor of apoptosis protein (IAP) family member, BmIAP, from Bombyx mori BmN cells. BmIAP contains two baculoviral IAP repeat (BIR) domains followed by a RING domain. BmIAP shares striking amino acid sequence similarity with lepidopteran IAPs, SfIAP and TnIAP, and with two baculoviral IAPs, CpIAP and OpIAP, suggesting evolutionary conservation. BmIAP blocks programmed cell death (apoptosis) in Spodoptera frugiperda Sf-21 cells induced by p35 deficient Autographa californica nucleopolyhedrovirus (AcMNPV). This anti-apoptotic function requires both the BIR domains and RING domain of BmIAP. In mammalian cells, BmIAP inhibits Bax induced but not Fas induced apoptosis. Further biochemical data suggest that BmIAP is a specific inhibitor of mammalian caspase-9, an initiator caspase in the mitochondria/cytochrome-c pathway, but not the downstream effector proteases, caspase-3 and caspase-7. These results suggest that suppression of apoptosis by lepidopteran IAPs in insect cells may involve inhibition of an upstream initiator caspase in the conserved mitochondria/cytochrome-c pathway for apoptosis. |
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Authors:
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Q Huang; Q L Deveraux; S Maeda; H R Stennicke; B D Hammock; J C Reed |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1499 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2001 Jan |
Date Detail:
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Created Date: 2001-05-08 Completed Date: 2001-05-21 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 191-8 Citation Subset: IM |
Affiliation:
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Department of Entomology, University of California, Davis 95616, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Apoptosis Baculoviridae / genetics Base Sequence Bombyx / genetics* Caspase 9 Caspases / antagonists & inhibitors, metabolism Cell Line Cloning, Molecular Cysteine Proteinase Inhibitors / metabolism DNA Primers / genetics Drosophila Proteins* Inhibitor of Apoptosis Proteins Insect Proteins / chemistry, genetics* Molecular Sequence Data Protein Structure, Tertiary Proto-Oncogene Proteins / metabolism Proto-Oncogene Proteins c-bcl-2* Spodoptera bcl-2-Associated X Protein |
| Grant Support | |
ID/Acronym/Agency:
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AG15402/AG/NIA NIH HHS; CA30199/CA/NCI NIH HHS; ES 05707/ES/NIEHS NIH HHS; ES02701/ES/NIEHS NIH HHS; ES04699/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cysteine Proteinase Inhibitors; 0/DIAP2 protein, Drosophila; 0/DNA Primers; 0/Drosophila Proteins; 0/Inhibitor of Apoptosis Proteins; 0/Insect Proteins; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; EC 3.4.22.-/Caspase 9; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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