Document Detail


Cloning and characterization of the hamster and guinea pig nicotinic acid receptors.
MedLine Citation:
PMID:  17602204     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, we present the identification and characterization of hamster and guinea pig nicotinic acid receptors. The hamster receptor shares approximately 80-90% identity with the nucleotide and amino acid sequences of human, mouse, and rat receptors. The guinea pig receptor shares 76-80% identity with the nucleotide and amino acid sequences of these other species. [(3)H]nicotinic acid binding affinity at guinea pig and hamster receptors is similar to that in human (dissociation constant = 121 nM for guinea pig, 72 nM for hamster, and 74 nM for human), as are potencies of nicotinic acid analogs in competition binding studies. Inhibition of forskolin-stimulated cAMP production by nicotinic acid and related analogs is also similar to the activity in the human receptor. Analysis of mRNA tissue distribution for the hamster and guinea pig nicotinic acid receptors shows expression across a number of tissues, with higher expression in adipose, lung, skeletal muscle, spleen, testis, and ovary.
Authors:
April Smith Torhan; Boonlert Cheewatrakoolpong; Lia Kwee; Scott Greenfeder
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Publication Detail:
Type:  Journal Article     Date:  2007-06-30
Journal Detail:
Title:  Journal of lipid research     Volume:  48     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-08-16     Completed Date:  2007-10-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2065-71     Citation Subset:  IM    
Affiliation:
Schering-Plough Research Institute, Department of Cardiovascular and Metabolic Diseases, Kenilworth, NJ 07033, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
CHO Cells
Cloning, Molecular
Cricetinae
Cricetulus
Guinea Pigs
Humans
Molecular Sequence Data
Niacin / metabolism
Receptors, Nicotinic / isolation & purification*,  metabolism*
Sequence Alignment
Chemical
Reg. No./Substance:
0/Receptors, Nicotinic; 59-67-6/Niacin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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