Document Detail


Cloning and characterization of the cDNA encoding human adenylosuccinate synthetase.
MedLine Citation:
PMID:  1592113     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adenylosuccinate synthetase (AS) catalyzes the first committed step in the conversion of IMP to AMP. A cDNA was isolated from a human liver library which encodes a protein of 455 amino acids (M(r) of 49,925). Alignments of human, mouse, Dictyostelium discoideum and E. coli AS sequences identify a number of invariant residues which are likely to be important for structure and/or catalysis. The human AS sequence was also 19% identical to the human urea cycle enzyme, argininosuccinate synthetase (ASS), which catalyzes a chemically similar reaction. Both human liver and HeLa AS mRNA showed signals of 2.3 and 2.8 kb. An unmodified N-terminus is required for function of the human AS enzyme in E. coli mutants lacking the bacterial enzyme. The human cDNA provides a means to assess the possible role of AS abnormalities in unclassified, idiopathic cases of gout.
Authors:
S M Powell; H Zalkin; J E Dixon
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  FEBS letters     Volume:  303     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1992 May 
Date Detail:
Created Date:  1992-06-30     Completed Date:  1992-06-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  4-10     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Purdue University, West Lafayette, IN 49707.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/S86390;  S86392;  S90294;  S90296;  S90298;  X65503;  X65504;  X66206;  X66503;  X71664
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MeSH Terms
Descriptor/Qualifier:
Adenylosuccinate Synthase / genetics*
Amino Acid Sequence
Base Sequence
Blotting, Northern
Cloning, Molecular
DNA / genetics*
Hela Cells
Humans
Liver / enzymology
Molecular Sequence Data
RNA, Messenger / genetics
Sequence Alignment
Grant Support
ID/Acronym/Agency:
GM 24658/GM/NIGMS NIH HHS; NIDDKD 18024/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Messenger; 9007-49-2/DNA; EC 6.3.4.4/Adenylosuccinate Synthase

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