Document Detail

Cloning and characterization of an alternative transcript of ovine glucose 6-phosphate dehydrogenase gene: comparative approach between ruminant and non-ruminant species.
MedLine Citation:
PMID:  17157446     Owner:  NLM     Status:  MEDLINE    
Glucose 6-phosphate dehydrogenase (G6PD) plays an important role in ruminant's lipogenesis, as it provides necessary compounds of NADPH for the synthesis of fatty acids catalyzing the first committed reaction in the pentose phosphate pathway. In this work the full length ovine glucose 6-phosphate dehydrogenase cDNA was isolated using a polymerase chain reaction based strategy. Two isoforms (OG6PDA and OG6PDB) were detected encoding a protein of 515 and 524 amino acids, respectively. Both deduced amino acid sequences reveal a well conserved protein containing all the important residues for its catalytic role. The extra nine amino acids encoded by OG6PDB cause a frameshift in the polypeptide chain resulting in changes around the area of the potential substrate binding site. A three-dimensional model of ovine G6PD protein shows that this frameshift cause structural changes in the catalytic binding "pocket" of the molecule. Southern blot and RT analysis revealed that ovine G6PD appears as a single copy gene while it is expressed, with slight variability, in all tissues analyzed. Moreover, expression analysis of the ovine G6PD isoforms showed that OG6PDB is expressed only in tissues where lipogenesis is high in ruminants. Thus, we hypothesize that in ruminants G6PD may be regulated by the ratio of the two transcripts, according to the existence stimulus.
George P Laliotis; Alexandros Argyrokastritis; Iosif Bizelis; Emmanuel Rogdakis
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-11-10
Journal Detail:
Title:  Gene     Volume:  388     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-01-02     Completed Date:  2007-03-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  93-101     Citation Subset:  IM    
Department of Animal Science, Laboratory of Animal Breeding and Husbandry, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/DQ377363;  DQ377364;  DQ890027
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MeSH Terms
Alternative Splicing*
Amino Acid Sequence
Base Sequence
Blotting, Southern
Catalytic Domain / genetics
Cloning, Molecular
DNA / chemistry,  genetics
DNA, Complementary / chemistry,  genetics
Gene Expression Profiling
Glucosephosphate Dehydrogenase / chemistry,  genetics*,  metabolism
Isoenzymes / chemistry,  genetics,  metabolism
Models, Molecular
Molecular Sequence Data
Protein Structure, Tertiary
Reverse Transcriptase Polymerase Chain Reaction
Ruminants / genetics
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Sheep / genetics*
Species Specificity
Reg. No./Substance:
0/DNA, Complementary; 0/Isoenzymes; 9007-49-2/DNA; EC Dehydrogenase

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