Document Detail


Cloning and characterization of acid invertase genes in the roots of the metallophyte Kummerowia stipulacea (Maxim.) Makino from two populations: Differential expression under copper stress.
MedLine Citation:
PMID:  24636951     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The roots of metallophytes serve as the key interface between plants and heavy metal-contaminated underground environments. It is known that the roots of metallicolous plants show a higher activity of acid invertase enzymes than those of non-metallicolous plants when under copper stress. To test whether the higher activity of acid invertases is the result of increased expression of acid invertase genes or variations in the amino acid sequences between the two population types, we isolated full cDNAs for acid invertases from two populations of Kummerowia stipulacea (from metalliferous and non-metalliferous soils), determined their nucleotide sequences, expressed them in Pichia pastoris, and conducted real-time PCR to determine differences in transcript levels during Cu stress. Heterologous expression of acid invertase cDNAs in P. pastoris indicated that variations in the amino acid sequences of acid invertases between the two populations played no significant role in determining enzyme characteristics. Seedlings of K. stipulacea were exposed to 0.3µM Cu(2+) (control) and 10µM Cu(2+) for 7 days under hydroponics׳ conditions. The transcript levels of acid invertases in metallicolous plants were significantly higher than in non-metallicolous plants when under copper stress. The results suggest that the expression of acid invertase genes in metallicolous plants of K. stipulacea differed from those in non-metallicolous plants under such conditions. In addition, the sugars may play an important role in regulating the transcript level of acid invertase genes and acid invertase genes may also be involved in root/shoot biomass allocation.
Authors:
Luan Zhang; Zhi-Ting Xiong; Zhong-Rui Xu; Chen Liu; Shen-Wen Cai
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-3-14
Journal Detail:
Title:  Ecotoxicology and environmental safety     Volume:  104C     ISSN:  1090-2414     ISO Abbreviation:  Ecotoxicol. Environ. Saf.     Publication Date:  2014 Mar 
Date Detail:
Created Date:  2014-3-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7805381     Medline TA:  Ecotoxicol Environ Saf     Country:  -    
Other Details:
Languages:  ENG     Pagination:  87-95     Citation Subset:  -    
Copyright Information:
Copyright © 2014 Elsevier Inc. All rights reserved.
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