| Cloning and characterization of SCIRR69: a novel transcriptional factor belonging to the CREB/ATF family. | |
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MedLine Citation:
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PMID: 22535319 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The complete cDNA sequence of a novel gene, SCIRR69 (spinal cord injury and regeneration related no. 69 gene), was obtained by RACE technique. It codes for a protein of 521 amino acid residues homologous to human CREB3l2 (also known as BBF2H7) and mouse CREB3l2. The protein contains a basic DNA binding and leucine zipper dimerization (B-ZIP) motif and a hydrophobic region representing a putative transmembrane domain, similar to the structure of other CREB/ATF transcription factors. Monoclonal antibody against SCIRR69 was developed and could recognize the SCIRR69 protein in both native and denatured forms. Constructing of SCIRR69 fusion proteins with the GAL4 DNA-binding domain disclosed that SCIRR69 functioned as a transcriptional activator and its N-terminal 60 amino acids accounted for the activation ability. SCIRR69 resides in the cytoplasm of primary neurons, whereas neuron damage by incision led to the cleavage and translocation from the cytoplasm to the nucleus. These results suggest that SCIRR69 is activated by proteolytic cleavage at the transmembrane domain in response to neuron damage and its amino-terminal cytoplasmic domain translocates into the nucleus to activate the transcription of target genes. |
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Authors:
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Zhenlian Ma; Haiping Que; Yanli Ni; Haiyan Huang; Yong Liu; Tao Liu; Xin Li; Qihong Sun; Shaojun Liu |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-4-26 |
Journal Detail:
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Title: Molecular biology reports Volume: - ISSN: 1573-4978 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-4-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0403234 Medline TA: Mol Biol Rep Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Institute of Basic Medical Sciences, 27 Taiping Road, Beijing, 100850, People's Republic of China. |
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