Document Detail


Cloning and characterization of CRF, a novel C1q-related factor, expressed in areas of the brain involved in motor function.
MedLine Citation:
PMID:  9878755     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have isolated and characterized a novel cDNA, C1q-Related Factor (CRF), that is predicted to encode a 258 amino acid polypeptide with a hydrophobic signal sequence, a collagenous region, and a globular domain at the carboxy terminus that shares homology to the C1q signature domain. Human CRF transcript is expressed at highest levels in the brain, particularly in the brainstem. In situ hybridization to mouse brain sections demonstrated that CRF transcripts are most abundant in areas of the nervous system involved in motor function, such as the Purkinje cells of the cerebellum, the accessory olivary nucleus, the pons and the red nucleus. The mouse CRF homolog is highly similar to the human gene at both the nucleotide and protein level, suggesting an important conserved role for this protein.
Authors:
N G Bérubé; X H Swanson; M J Bertram; J D Kittle; V Didenko; D S Baskin; J R Smith; O M Pereira-Smith
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Brain research. Molecular brain research     Volume:  63     ISSN:  0169-328X     ISO Abbreviation:  Brain Res. Mol. Brain Res.     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-02-19     Completed Date:  1999-02-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8908640     Medline TA:  Brain Res Mol Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  233-40     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Elsevier Science B.V.
Affiliation:
Roy M. and Phyllis Gough Huffington Center on Aging, Division of Molecular Virology, Departments of Cell Biology and Medicine, Baylor College of Medicine, One Baylor Plaza (M-320), Houston, TX 77030-3498, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Brain Chemistry / physiology*
Cloning, Molecular
Complement Activation*
Complement C1q / isolation & purification*
Humans
Mice
Molecular Sequence Data
Motor Activity / physiology*
Protein Biosynthesis
RNA / biosynthesis
Sequence Homology, Amino Acid
Grant Support
ID/Acronym/Agency:
P01AG13663/AG/NIA NIH HHS; R37AG05333/AG/NIA NIH HHS; T32AG00183/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
63231-63-0/RNA; 80295-33-6/Complement C1q

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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