Document Detail


Cloning and characterization of a novel NAD(+) -dependent glyceraldehyde-3-phosphate dehydrogenase gene from Candida glycerinogenes and use of its promoter.
MedLine Citation:
PMID:  23420642     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A 3950 bp genomic fragment from Candida glycerinogenes, WL2002-5, containing the CgGAP gene encoding a glyceraldehyde-3-phosphate dehydrogenase homologous to GAP genes in other yeasts using degenerate primers, was cloned and characterized with inverse PCR. Sequence analysis revealed a 1164 bp open reading frame encoding a putative peptide of 387 deduced amino acids, with a molecular mass of 36 kDa. The CgGAP protein consisted of an N-terminal NAD(+) -binding domain and a central catalytic domain. Six stress-response elements were found in the upstream region of the CgGAP gene. The influence of CgGAP on glycolysis was investigated. Functional analysis revealed that Saccharomyces cerevisiae transformed with CgGAP was restored to the wild-type phenotype when cultured in high-osmolarity medium, suggesting that it is a functional GAP protein. Promoter studies in S. cerevisiae using the green fluorescent protein (gfp) gene as a reporter showed that the GAP promoter (PCgGAP ) is constitutively expressed in S. cerevisiae cells grown on glucose.
Authors:
Cheng Zhang; Bin Zhuge; Xiaobei Zhan; Huiying Fang; Hong Zong; Jian Zhuge
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-03-12
Journal Detail:
Title:  Yeast (Chichester, England)     Volume:  30     ISSN:  1097-0061     ISO Abbreviation:  Yeast     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-04-12     Completed Date:  2013-09-30     Revised Date:  2013-10-18    
Medline Journal Info:
Nlm Unique ID:  8607637     Medline TA:  Yeast     Country:  England    
Other Details:
Languages:  eng     Pagination:  157-63     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 John Wiley & Sons, Ltd.
Affiliation:
Key Laboratory of Industrial Biotechnology of Ministry of Education, Research Centre of Industrial Microorganisms, School of Biotechnology, Jiangnan University, Wuxi, 214122, P.R., China.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Candida / classification,  enzymology*,  genetics
Cloning, Molecular
Fungal Proteins / genetics*,  metabolism
Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / genetics*,  metabolism
Molecular Sequence Data
Phylogeny
Promoter Regions, Genetic*
Chemical
Reg. No./Substance:
0/Fungal Proteins; EC 1.2.1.12/Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)

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