Document Detail

Cloning of a centromere binding factor 3d (CBF3D) gene from Candida glabrata.
MedLine Citation:
PMID:  10398347     Owner:  NLM     Status:  MEDLINE    
The gene encoding centromere binding factor 3d (CBF3D) of the human pathogenic yeast Candida glabrata has been isolated by hybridization of Saccharomyces cerevisiae CBF3D (ScCBF3D) DNA to a C. glabrata partial genomic library. Sequence analysis revealed a 540 bp open reading frame encoding a protein of 179 amino acids with a calculated molecular mass of 20.9 kDa. The amino acid sequence is highly homologous (78.6% identity) to ScCbf3d and 48.3% identical to the human homologue p19 (SKP1). Southern blot analysis indicates that CgCbf3d is encoded by an unique nuclear gene. The cloned CgCBF3D gene can functionally substitute the S. cerevisiae homologue in a S. cerevisiae CBF3D-deletion mutant. The GenBank Accession No. for this gene is AF 072472.
T Stoyan; R Eck; J Lechner; P Hemmerich; W Künkel; S Diekmann
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Yeast (Chichester, England)     Volume:  15     ISSN:  0749-503X     ISO Abbreviation:  Yeast     Publication Date:  1999 Jun 
Date Detail:
Created Date:  1999-08-12     Completed Date:  1999-08-12     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8607637     Medline TA:  Yeast     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  793-8     Citation Subset:  IM    
Copyright Information:
Copyright 1999 John Wiley & Sons, Ltd.
Institut für Molekulare Biotechnologie, Abt. Molekularbiologie, Beutenbergstrasse 11, 07745 Jena, Germany.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Candida / genetics*
Cloning, Molecular
Cross Reactions
DNA-Binding Proteins / chemistry,  genetics*,  physiology
F-Box Proteins*
Gene Dosage
Genetic Complementation Test
Genomic Library
Molecular Sequence Data
Molecular Weight
Open Reading Frames / genetics
SKP Cullin F-Box Protein Ligases*
Saccharomyces cerevisiae / genetics,  growth & development
Saccharomyces cerevisiae Proteins*
Sequence Deletion
Sequence Homology, Amino Acid
Reg. No./Substance:
0/DNA-Binding Proteins; 0/F-Box Proteins; 0/SKP1 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; EC Cullin F-Box Protein Ligases

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