Document Detail

Cloning of canine osteosarcoma cells.
MedLine Citation:
PMID:  7756419     Owner:  NLM     Status:  MEDLINE    
Six clonal cells were established from a canine osteosarcoma cell line (POS) by a limiting dilution method using feeder cells. Whereas histology of mass developed in nude mice by transplantation of POS cells revealed various cell types, clonal cells were morphologically consistent in size and shape. Doubling time of clonal cells ranged from 30 +/- 1.4 to 54 +/- 1.3 hr and alkaline phosphatase activity ranged from 0.040 +/- 0.001 to 2.61 +/- 0.435 mumol/min/mg protein depending on the cell types. When transplanted into nude mice, each clonal cell type formed following four histological types; osteoblastic, chondroblastic, fibroblastic, and undifferentiated types. Since each histological feature was found simultaneously in the primary tumor, osteosarcoma tissue might be a complex of various types of cells having different characteristics. Therefore, POS clonal cells may be useful as a potential tool for the studies of differentiation, phenotypic expression, and a new therapeutic modality of osteosarcoma.
K Nozaki; K Okuyama; T Kadosawa; H Nakayama; N Goto; N Sasaki; A Takeuchi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of veterinary medical science / the Japanese Society of Veterinary Science     Volume:  57     ISSN:  0916-7250     ISO Abbreviation:  J. Vet. Med. Sci.     Publication Date:  1995 Feb 
Date Detail:
Created Date:  1995-06-26     Completed Date:  1995-06-26     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9105360     Medline TA:  J Vet Med Sci     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  23-8     Citation Subset:  IM    
Department of Veterinary Surgery, Faculty of Agriculture, University of Tokyo, Japan.
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MeSH Terms
Alkaline Phosphatase / metabolism
Clone Cells / pathology*
Dog Diseases / enzymology,  pathology*
Mice, Inbred BALB C
Mice, Nude
Neoplasm Transplantation
Osteosarcoma / enzymology,  pathology,  veterinary*
Specific Pathogen-Free Organisms
Tumor Cells, Cultured / pathology*
Reg. No./Substance:
EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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