Document Detail


Cloning of canine IL-1ra, TNFR and TIMP-2.
MedLine Citation:
PMID:  11182158     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This paper describes the cloning and sequence analysis of the cDNA's encoding the canine homologues of interleukin-1 receptor antagonist (IL-1ra), tumour necrosis factor receptor extra-cellular domain (TNFR/ECD) and tissue inhibitor of metalloproteinase-2 (TIMP-2). The coding sequences for canine IL-1ra and TNFR/ECD were obtained using reverse transcription polymerase chain reaction (RT-PCR) using RNA harvested from canine peripheral blood mononuclear cells (PBMC) and TIMP-2 was isolated in a similar fashion from the canine D17 osteosarcoma cell line. Sequence analysis of the canine genes demonstrated open reading frames of 531, 633 and 663 base pairs (bp), respectively. All three canine proteins IL-1ra, TNFR/ECD and TIMP-2 (177, 211 and 221 amino acids, respectively) showed considerable sequence similarity with the homologous sequences published for other species.
Authors:
S E Campbell; L Nasir; D J Argyle; E A Gault; S Duthie; D Bennett
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Veterinary immunology and immunopathology     Volume:  78     ISSN:  0165-2427     ISO Abbreviation:  Vet. Immunol. Immunopathol.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-05-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8002006     Medline TA:  Vet Immunol Immunopathol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  207-14     Citation Subset:  IM    
Affiliation:
Molecular Therapeutics Research Group, Department of Clinical Studies, Faculty of Veterinary Medicine, University of Glasgow, Bearsden Road, G61 1HQ, Glasgow, UK. 9367131t@student.gla.ac.uk
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF013955;  AF112115;  AF216526
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
DNA Primers / chemistry
DNA, Complementary / chemistry,  genetics
Dogs / genetics*
Humans
Interleukin 1 Receptor Antagonist Protein
Molecular Sequence Data
RNA / chemistry,  isolation & purification
Receptors, Interleukin-1 / antagonists & inhibitors,  chemistry,  genetics
Receptors, Tumor Necrosis Factor / chemistry,  genetics*
Reverse Transcriptase Polymerase Chain Reaction / veterinary
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Sialoglycoproteins / chemistry,  genetics*
Tissue Inhibitor of Metalloproteinase-2 / chemistry,  genetics*
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Complementary; 0/IL1RN protein, human; 0/Interleukin 1 Receptor Antagonist Protein; 0/Receptors, Interleukin-1; 0/Receptors, Tumor Necrosis Factor; 0/Sialoglycoproteins; 127497-59-0/Tissue Inhibitor of Metalloproteinase-2; 63231-63-0/RNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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