| Cloning of cDNAs coding for rat hepatic microsomal UDP-glucuronyltransferases. | |
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MedLine Citation:
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PMID: 2989105 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Radioiodinated, affinity-purified, anti-UDP-glucuronyltransferase (UDPGT) antibodies have been used to isolate cDNAs coding for UDPGT(s) from a rat liver cDNA library cloned in the expression vector bacteriophage lambda gt11. The sizes of ten cloned cDNAs range from 0.3-2.1 kb. The identity of the cDNAs was confirmed by the hybrid-select translation and immunochemical analyses. Restriction mapping indicates that two classes of cDNA coding for different UDPGT mRNAs have been cloned. |
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Authors:
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M R Jackson; L R McCarthy; R B Corser; G C Barr; B Burchell |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Gene Volume: 34 ISSN: 0378-1119 ISO Abbreviation: Gene Publication Date: 1985 |
Date Detail:
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Created Date: 1985-08-21 Completed Date: 1985-08-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7706761 Medline TA: Gene Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 147-53 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cloning, Molecular DNA / genetics DNA Restriction Enzymes / diagnostic use DNA, Recombinant Glucuronosyltransferase / genetics* Microsomes, Liver / enzymology* Molecular Weight Protein Biosynthesis RNA, Messenger / genetics Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
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0/DNA, Recombinant; 0/RNA, Messenger; 9007-49-2/DNA; EC 2.4.1.17/Glucuronosyltransferase; EC 3.1.21.-/DNA Restriction Enzymes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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