| Cloning of cDNA encoding rat spermatidal protein TP2 and expression in Escherichia coli. | |
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MedLine Citation:
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PMID: 8954887 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rat spermatidal protein TP2 is a basic nuclear protein containing two atoms of zinc bound per molecule. We report here cloning of complementary DNA encoding rat TP2 by the RT-PCR method. The nucleotide sequence of cloned TP2 cDNA differs at a few positions from the sequence already reported in the literature. We have cloned rat TP2 cDNA into the expression vector pTrc 99A. Upon induction with 1 mM IPTG, there was a low level of expression of TP2 which could be recovered in the soluble form. Recombinant TP2 was purified from the soluble form. Recombinant TP2 was purified from the soluble extract of E. coli using nickel-agarose and heparin-agarose chromatography and was shown to be identical to native rat TP2 as revealed by immunoblotting with anti-rat TP2 antibodies and radioactive 65Zn-blotting. |
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Authors:
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A R Meetei; M R Rao |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Protein expression and purification Volume: 8 ISSN: 1046-5928 ISO Abbreviation: Protein Expr. Purif. Publication Date: 1996 Dec |
Date Detail:
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Created Date: 1997-03-18 Completed Date: 1997-03-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9101496 Medline TA: Protein Expr Purif Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 409-15 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Indian Institute of Science, Bangalore, India. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/U52958 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Blotting, Western Chromosomal Proteins, Non-Histone / genetics* Cloning, Molecular DNA, Complementary / chemistry Electrophoresis, Polyacrylamide Gel Escherichia coli Humans Male Molecular Sequence Data Polymerase Chain Reaction Rats Zinc |
| Chemical | |
Reg. No./Substance:
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0/Chromosomal Proteins, Non-Histone; 0/DNA, Complementary; 0/spermatid transition proteins; 7440-66-6/Zinc |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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