Document Detail

Cloning of bovine MAIL and its mRNA expression in white blood cells of Holstein cows.
MedLine Citation:
PMID:  15010226     Owner:  NLM     Status:  MEDLINE    
Molecule possessing ankyrin-repeats induced by lipopolysaccharide (MAIL) is known as an IkappaB protein induced after administration of bacterial lipopolysaccharide (LPS) to mice. In the present study, we cloned bovine MAIL cDNA and examined its mRNA expression in white blood cells isolated from Holstein cows. Bovine MAIL had more than 80% amino acid identities with murine and human MAILs, highly conserved ankyrin-repeat motifs and PEST-like sequences. Bovine MAIL mRNA was undetectable in isolated peripheral white blood cells, but rapidly induced (<1h) after stimulation by LPS and lipid A in vitro in a dose-dependent manner. The lipid A-induced MAIL mRNA expression was found in polymorphonuclear cells, monocytes/macrophages and total lymphocytes, but not in T-lymphocytes. MAIL mRNA was also induced in vivo in peripheral blood leukocytes of cows after intramammary injection of Escherichia coli derived from coliform mastitis. Thus, bovine MAIL, as rodent MAILs, is induced by inflammatory stimuli in specific immune cells in vitro and in vivo, suggesting a role in inflammatory responses to bacterial infection in cattle.
Daisuke Yamaji; Hiroshi Kitamura; Kazuhiro Kimura; Yukiko Matsushita; Hiroyuki Okada; Takahiko Shiina; Masami Morimatsu; Masayuki Saito
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Veterinary immunology and immunopathology     Volume:  98     ISSN:  0165-2427     ISO Abbreviation:  Vet. Immunol. Immunopathol.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-03-10     Completed Date:  2004-05-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8002006     Medline TA:  Vet Immunol Immunopathol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  175-84     Citation Subset:  IM    
Laboratory of Biochemistry, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
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MeSH Terms
Amino Acid Motifs
Amino Acid Sequence
Base Sequence
Blotting, Northern / veterinary
Cattle / immunology*
Cloning, Molecular
Conserved Sequence
Gene Expression Regulation / immunology
Leukocytes, Mononuclear / immunology
Molecular Sequence Data
Nuclear Proteins / biosynthesis,  genetics*,  immunology
RNA, Messenger / biosynthesis*,  genetics
Reverse Transcriptase Polymerase Chain Reaction / veterinary
Sequence Alignment
Sequence Analysis, DNA
Reg. No./Substance:
0/Nuclear Proteins; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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