Document Detail


Cloning of an arylalkylamine N-acetyltransferase (aaNAT1) from Drosophila melanogaster expressed in the nervous system and the gut.
MedLine Citation:
PMID:  8901578     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In insects, neurotransmitter catabolism, melatonin precursor formation, and sclerotization involve arylalkylamine N-acetyltransferase (aaNAT, EC 2.3.1.87) activity. It is not known if one or multiple aaNAT enzymes are responsible for these activities. We recently have purified an aaNAT from Drosophila melanogaster. Here, we report the cloning of the corresponding aaNAT cDNA (aaNAT1) that upon COS cell expression acetylates dopamine, tryptamine, and the immediate melatonin precursor serotonin. aaNAT1 represents a novel gene family unrelated to known acetyl-transferases, except in two weakly conserved amino acid motifs. In situ hybridization studies of aaNAT1 mRNA in embryos reveal hybridization signals in the brain, the ventral cord, the gut, and probably in oenocytes, indicating a broad tissue distribution of aaNAT1 transcripts. Moreover, in day/ night studies we demonstrate a diurnal rhythm of melatonin concentration without a clear-cut change in aaNAT1 mRNA levels. The data suggest that tissue-specific regulation of aaNAT1 may be associated with different enzymatic functions and do not exclude the possibility of additional aaNAT genes.
Authors:
E Hintermann; N C Grieder; R Amherd; D Brodbeck; U A Meyer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  93     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1996-12-24     Completed Date:  1996-12-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  12315-20     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, University of Basel, Switzerland.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/Y07964
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MeSH Terms
Descriptor/Qualifier:
Acetylation
Acetyltransferases / genetics
Amino Acid Sequence
Animals
Arylamine N-Acetyltransferase / genetics*,  metabolism
Blotting, Southern
COS Cells
Chromosomes / chemistry
Circadian Rhythm
Cloning, Molecular
Conserved Sequence
Digestive System / enzymology
Drosophila melanogaster
Humans
In Situ Hybridization
Kinetics
Mice
Molecular Sequence Data
Nervous System / enzymology
RNA, Messenger / metabolism
Restriction Mapping
Xenopus
Chemical
Reg. No./Substance:
0/RNA, Messenger; EC 2.3.1.-/Acetyltransferases; EC 2.3.1.5/Arylamine N-Acetyltransferase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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