| Cloning of an arylalkylamine N-acetyltransferase (aaNAT1) from Drosophila melanogaster expressed in the nervous system and the gut. | |
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MedLine Citation:
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PMID: 8901578 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In insects, neurotransmitter catabolism, melatonin precursor formation, and sclerotization involve arylalkylamine N-acetyltransferase (aaNAT, EC 2.3.1.87) activity. It is not known if one or multiple aaNAT enzymes are responsible for these activities. We recently have purified an aaNAT from Drosophila melanogaster. Here, we report the cloning of the corresponding aaNAT cDNA (aaNAT1) that upon COS cell expression acetylates dopamine, tryptamine, and the immediate melatonin precursor serotonin. aaNAT1 represents a novel gene family unrelated to known acetyl-transferases, except in two weakly conserved amino acid motifs. In situ hybridization studies of aaNAT1 mRNA in embryos reveal hybridization signals in the brain, the ventral cord, the gut, and probably in oenocytes, indicating a broad tissue distribution of aaNAT1 transcripts. Moreover, in day/ night studies we demonstrate a diurnal rhythm of melatonin concentration without a clear-cut change in aaNAT1 mRNA levels. The data suggest that tissue-specific regulation of aaNAT1 may be associated with different enzymatic functions and do not exclude the possibility of additional aaNAT genes. |
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Authors:
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E Hintermann; N C Grieder; R Amherd; D Brodbeck; U A Meyer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 93 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1996 Oct |
Date Detail:
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Created Date: 1996-12-24 Completed Date: 1996-12-24 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 12315-20 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, University of Basel, Switzerland. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/Y07964 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetylation Acetyltransferases / genetics Amino Acid Sequence Animals Arylamine N-Acetyltransferase / genetics*, metabolism Blotting, Southern COS Cells Chromosomes / chemistry Circadian Rhythm Cloning, Molecular Conserved Sequence Digestive System / enzymology Drosophila melanogaster Humans In Situ Hybridization Kinetics Mice Molecular Sequence Data Nervous System / enzymology RNA, Messenger / metabolism Restriction Mapping Xenopus |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; EC 2.3.1.-/Acetyltransferases; EC 2.3.1.5/Arylamine N-Acetyltransferase |
| Comments/Corrections | |
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