| Cloning of Small RNAs for the Discovery of Novel MicroRNAs in Plants. | |
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MedLine Citation:
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PMID: 23135848 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Endogenous small RNAs can be grouped into several distinct classes of 21-nt-long microRNAs (miRNAs), short interfering RNAs (siRNAs), trans-acting siRNAs (tasiRNAs), and 24-nt long heterochromatic siRNAs. miRNAs are increasingly being recognized as significant effectors of gene regulation in a wide range of organisms. These molecules are typically ∼21-nt long and are amenable for cloning by streamlined protocols. Here we detail the methodology for cloning small RNAs in rice to identify novel miRNAs and other important small RNAs. Briefly, small RNA molecules are size fractionated, attached to adaptors, and subsequently converted into cDNA and PCR amplified. Current high-throughput sequencing technologies allow sequencing of the PCR products directly. |
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Authors:
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Guru Jagadeeswaran; Ramanjulu Sunkar |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 956 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-11-08 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 109-18 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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