Document Detail


Cloning, expression and characterization of recombinant exotoxin A-flagellin fusion protein as a new vaccine candidate against Pseudomonas aeruginosa infections.
MedLine Citation:
PMID:  23279828     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Infections due to Pseudomonas aeruginosa are among the leading causes of morbidity and mortality in patients who suffer from impaired immune responses and chronic diseases such as cystic fibrosis. At present, aggressive antibiotic therapy is the only choice for management of P. aeruginosa infections, but emergence of highly resistant strains necessitated the development of novel alternative therapeutics including an effective vaccine. Several P. aeruginosa antigens have been tested for vaccine development, including lipopolysaccharide alone, polysaccharides alginate, extracellular proteins, exotoxin A (exo A) and killed whole cell. However, none of them are currently available clinically.
METHODS: In this research, recombinant exoA-flagellin (fliC) fusion protein as a cocktail antigen was expressed and purified and its antigenic characteristics were evaluated.
RESULTS: Expression of recombinant fusion protein by E. coli using pET22b vector resulted in production of exoA-fliC fusion protein in high concentration. Based on Western-blotting results, recombinant fusion protein showed a good antigenic interaction with sera from patients with various P. aeruginosa infections.
CONCLUSION: These results suggested that recombinant exoA-fliC fusion protein can be produced in the laboratory, and tested as a candidate vaccine in P. aeruginosa infections.
Authors:
Asghar Tanomand; Safar Farajnia; Shahin Najar Peerayeh; Jafar Majidi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Iranian biomedical journal     Volume:  17     ISSN:  2008-823X     ISO Abbreviation:  Iran. Biomed. J.     Publication Date:  2013  
Date Detail:
Created Date:  2013-01-02     Completed Date:  2013-06-25     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  9814853     Medline TA:  Iran Biomed J     Country:  Iran    
Other Details:
Languages:  eng     Pagination:  1-7     Citation Subset:  IM    
Affiliation:
Dept. of Bacteriology Faculty of Medical sciences, Tarbiat Modares University, Tehran, Iran.
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MeSH Terms
Descriptor/Qualifier:
ADP Ribose Transferases / genetics,  immunology*
Antibodies, Bacterial / blood,  immunology
Bacterial Proteins / genetics,  immunology
Bacterial Toxins / genetics,  immunology*
Bacterial Vaccines* / genetics,  immunology
Cloning, Molecular
Escherichia coli / genetics
Exotoxins / genetics,  immunology*
Flagellin / genetics,  immunology*
Humans
Nucleic Acid Amplification Techniques
Pseudomonas Infections / immunology,  prevention & control*,  therapy
Pseudomonas aeruginosa / genetics,  immunology
Recombinant Fusion Proteins / genetics*,  immunology*
Sequence Analysis, DNA
Vaccines, Synthetic / genetics,  immunology
Virulence Factors / genetics,  immunology*
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Bacterial Proteins; 0/Bacterial Toxins; 0/Bacterial Vaccines; 0/Exotoxins; 0/Recombinant Fusion Proteins; 0/Vaccines, Synthetic; 0/Virulence Factors; 12777-81-0/Flagellin; EC 2.4.2.-/ADP Ribose Transferases; EC 2.4.2.31/toxA protein, Pseudomonas aeruginosa
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