| Cloning, expression and characterization of Mycobacterium tuberculosis lipoprotein LprF. | |
| | |
MedLine Citation:
|
PMID: 19944079 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Lipoproteins are well known virulence factors of bacterial pathogens in general and of Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis, in particular. Lipoprotein lipidation between Gram-positive and Gram-negative bacteria differs significantly as these are di- and triacylated, respectively. Little is known about the lipid anchor of mycobacterial lipoproteins. We reported recently that mycobacterial LppX, a lipoprotein involved in synthesis of cell wall components is triacylated, although mycobacteria are classified as GC-rich Gram-positive bacteria. We here exploited the model organism Mycobacterium smegmatis for the expression of Mtb LprF and characterized N-terminal modifications at the molecular level. LprF is a putative lipoprotein of Mtb involved in signaling of potassium-dependent osmotic stress. LprF is extensively modified in a mycobacterium-specific manner by a thioether-linked diacylglyceryl residue with one ester-bound tuberculostearic- and one C16:0 fatty acid and additionally by a third N-linked C16:0 fatty acid, and a hexose. |
| | |
Authors:
|
Juliane K Br?lle; Thomas Grau; Andreas Tschumi; Yolanda Auchli; Reto Burri; Silke Polsfuss; Peter M Keller; Peter Hunziker; Peter Sander |
Related Documents
:
|
8521599 - Sialic acid and oxidizability of low density lipoprotein subfractions of hyperlipidemic... 8633679 - Fasting serum triglycerides, free fatty acids, and malondialdehyde are increased in pre... 2951469 - Isolation and characterization of the two major apoproteins in human lipoprotein [a]. 8477699 - Oxygenation of lipoproteins by mammalian lipoxygenases. 8136359 - Hydration and localization of diacylglycerol in the insect lipoprotein lipophorin. a 13... 7052069 - Insulin and non-esterified fatty acids. acute regulators of lipogenesis in perfused rat... 11318539 - Standard acid reflux testing revisited. 16325749 - Linoleic acid, but not oleic acid, upregulates production of interleukin-8 by human vas... 18162709 - Meta-analysis of the association between hla-drb1 allele and rheumatoid arthritis susce... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-26 |
Journal Detail:
|
Title: Biochemical and biophysical research communications Volume: 391 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2010 Jan |
Date Detail:
|
Created Date: 2010-01-27 Completed Date: 2010-03-15 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
|
Languages: eng Pagination: 679-84 Citation Subset: IM |
Copyright Information:
|
Copyright 2009 Elsevier Inc. All rights reserved. |
Affiliation:
|
Institute of Medical Microbiology, University of Zurich, Gloriastr 30/32, CH-8006 Zurich, Switzerland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Lipoproteins / biosynthesis, chemistry*, genetics Molecular Sequence Data Mycobacterium smegmatis / metabolism Mycobacterium tuberculosis / metabolism* Recombinant Proteins / biosynthesis, chemistry, genetics Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| Chemical | |
Reg. No./Substance:
|
0/Lipoproteins; 0/Recombinant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: 1.45 A resolution crystal structure of recombinant PNP in complex with a pM multisubstrate analogue ...
Next Document: Optical tracking of phenotypically diverse individual synapses on solitary tract nucleus neurons.