Document Detail

Cloning, expression and characterization of Mycobacterium tuberculosis lipoprotein LprF.
MedLine Citation:
PMID:  19944079     Owner:  NLM     Status:  MEDLINE    
Lipoproteins are well known virulence factors of bacterial pathogens in general and of Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis, in particular. Lipoprotein lipidation between Gram-positive and Gram-negative bacteria differs significantly as these are di- and triacylated, respectively. Little is known about the lipid anchor of mycobacterial lipoproteins. We reported recently that mycobacterial LppX, a lipoprotein involved in synthesis of cell wall components is triacylated, although mycobacteria are classified as GC-rich Gram-positive bacteria. We here exploited the model organism Mycobacterium smegmatis for the expression of Mtb LprF and characterized N-terminal modifications at the molecular level. LprF is a putative lipoprotein of Mtb involved in signaling of potassium-dependent osmotic stress. LprF is extensively modified in a mycobacterium-specific manner by a thioether-linked diacylglyceryl residue with one ester-bound tuberculostearic- and one C16:0 fatty acid and additionally by a third N-linked C16:0 fatty acid, and a hexose.
Juliane K Br?lle; Thomas Grau; Andreas Tschumi; Yolanda Auchli; Reto Burri; Silke Polsfuss; Peter M Keller; Peter Hunziker; Peter Sander
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-26
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  391     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-01-27     Completed Date:  2010-03-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  679-84     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier Inc. All rights reserved.
Institute of Medical Microbiology, University of Zurich, Gloriastr 30/32, CH-8006 Zurich, Switzerland.
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MeSH Terms
Amino Acid Sequence
Lipoproteins / biosynthesis,  chemistry*,  genetics
Molecular Sequence Data
Mycobacterium smegmatis / metabolism
Mycobacterium tuberculosis / metabolism*
Recombinant Proteins / biosynthesis,  chemistry,  genetics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Reg. No./Substance:
0/Lipoproteins; 0/Recombinant Proteins

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