| Cloning, Expression and Characterization of Mouse (Mus musculus) Nicotinamide 5'-Mononucleotide Adenylyltransferase-2. | |
| | |
MedLine Citation:
|
PMID: 22313312 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Nicotinamide adenine dinucleotide (NAD+) is synthesized by the action of nicotinamide mononucleotide adenylyltransferase (NMNAT) from NMN and ATP. The mouse homolog of NMNAT-2 (mmNMNAT-2) was cloned, expressed, and subsequently identified using MALDI-TOF in conjunction with the ProFound database. Circular dichroism analyses of recombinant mmNMNAT-2 showed α helical and β sheet secondary structures, consistent with the known structure of the human isoform. Competition experiments using mouse pancreatic tissue lysates with recombinant mmNMNAT-2 demonstrated that the activity of the expressed protein was similar to the human isoform. Immunohistochemistry of mouse embryonic tissues with hNMNAT-2 also showed a tissue- and cellular-specific expression of this isoform. Therefore, our studies demonstrate for the first time the clear biological evidence for the existence of a mouse isoform of hNMNAT-2. These studies may help in future investigations aimed at understanding the regulation of this gene and its pathway, and in turn, will spur the development of novel therapies for diseases such as cancer and diabetes since mice are the most frequently used experimental system for in vivo studies. |
| | |
Authors:
|
David W Raches; Suhong Xiao; Praveen Kusumanchi; Joel Yalowitz; Paresh Sanghani; Eric C Long; Asok C Antony; Hiremagalur N Jayaram |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Medicinal chemistry (Shāriqah (United Arab Emirates)) Volume: 7 ISSN: 1875-6638 ISO Abbreviation: Med Chem Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2012-02-08 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101240303 Medline TA: Med Chem Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 718-26 Citation Subset: IM |
Affiliation:
|
Richard L. Roudebush Veterans Affairs Medical Center-151, 1481 West Tenth street, Indianapolis, Indiana, 46202, USA. hjayaram@iupui.edu. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Novel Hexahydropyridoindole Derivative as Prospective Agent Against Oxidative Damage in Pancreatic ?...
Next Document: Synthesis and Evaluation of Biological Activities of a Series of (6-substitutedbenzothiazol-2-yl)acr...