Document Detail

Cloning of Cyc (Bmal1) homolog in Bombyx mori: structural analysis and tissue specific distributions.
MedLine Citation:
PMID:  12628385     Owner:  NLM     Status:  MEDLINE    
Cycle (Bmal1) is one of the circadian clock genes and the key regulator of the circadian system in many organisms, encoding a bHLH-PAS transcription factor. In the present study, we cloned cycle homolog (BmCyc) in Bombyx mori. We performed polymerase chain reaction with degenerated primers deduced from the conserved amino acid sequences of mammalian BMAL1 and Drosophila CYCLE. Then the partial clone obtained was used as a probe for screening a cDNA library constructed from pupal brains of B. mori. BmCyc is 5703 nucleotides long and encodes 700 amino acid residues. The BmCYC has bHLH, PAS A and PAS B domains, and the sequence identities for these domains are 85, 60 and 50%, to Drosophila CYCLE (dCYC) and 69, 58 and 50%, to human BMAL1 (hBMAL1), respectively. The deduced amino acid sequence of BmCYC is 37% identical to that of dCYC and 28% to hBMAL1. Northern blot analysis demonstrates that BmCyc gene was expressed in all the tissues tested, which were head, fat body, silk glands, and midgut. Also no significant day, night time-specific difference in expression of BmCyc gene in the head was detected.
Elitza P Markova; Hitoshi Ueda; Katsuhiko Sakamoto; Katsutaka Oishi; Toru Shimada; Makio Takeda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology     Volume:  134     ISSN:  1096-4959     ISO Abbreviation:  Comp. Biochem. Physiol. B, Biochem. Mol. Biol.     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-03-11     Completed Date:  2003-12-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9516061     Medline TA:  Comp Biochem Physiol B Biochem Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  535-42     Citation Subset:  IM    
Division of Molecular Science, Graduate School of Science and Technology, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Blotting, Northern
Bombyx / genetics*
Circadian Rhythm / genetics
Cloning, Molecular
Conserved Sequence / genetics
DNA, Complementary / chemistry,  genetics
Gene Expression
Gene Library
Insect Proteins / genetics*
Molecular Sequence Data
RNA, Messenger / genetics,  metabolism
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Time Factors
Transcription Factors / genetics*,  metabolism
Reg. No./Substance:
0/DNA, Complementary; 0/Insect Proteins; 0/RNA, Messenger; 0/Transcription Factors

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