Document Detail

Cloning and 5'-flanking sequence of a rat cholesterol 7 alpha-hydroxylase gene.
MedLine Citation:
PMID:  1420318     Owner:  NLM     Status:  MEDLINE    
A cholesterol 7 alpha-hydroxylase gene containing 8 kb of the 5'-flanking region and 5 kb of the transcription region which covers exons 1 to 5 was isolated from a rat genomic library. The 2015 bp nucleotide sequence 5'-upstream from the start codon was determined. This promotor region contains many liver-enriched or -specific elements (TGT3, HNF/LF-B1), putative hormone responsive elements (TRE, GRE, RRE or RARE) and ubiquitous transcription factor binding sites (NF-1, OCT-1). In addition, 21 CA repeats which have potential to form the Z-DNA structure were found in this region. These putative regulatory elements and repetitive motifs may play roles in the regulation of cholesterol 7 alpha-hydroxylase gene in the liver. The sequence identity of the rat gene to the human gene in this region is low. Only liver-enriched elements are conserved in the cholesterol 7 alpha-hydroxylase genes.
J Y Chiang; T P Yang; D P Wang
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1132     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1992 Oct 
Date Detail:
Created Date:  1992-12-16     Completed Date:  1992-12-16     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  337-9     Citation Subset:  IM    
Department of Biochemistry and Molecular Pathology, Northeastern Ohio Universities College of Medicine, Rootstown 44272.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/L07316;  M91429;  M91430;  M93297;  M93298;  M93299;  M93300;  M93301;  M94056;  Z14108
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cholesterol 7-alpha-Hydroxylase / genetics*
Cloning, Molecular
Molecular Sequence Data
Promoter Regions, Genetic*
Restriction Mapping
Grant Support
Reg. No./Substance:
9007-49-2/DNA; EC 7-alpha-Hydroxylase

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