Document Detail

Cloning of 5'-flanking region and a polymorphic CTT trinucleotide repeat within 5'-untranslated region of mouse R-type calcium channel alpha1-subunit (Cchra1) gene, and its genetic mapping.
MedLine Citation:
PMID:  9651528     Owner:  NLM     Status:  MEDLINE    
The 5'-flanking region of the mouse R-type calcium channel (Cchra1) gene was cloned, and a transcriptional start point (tsp) was determined by rapid amplification of 5'-cDNA end (5'RACE) method. The putative promoter region of the gene contained no obvious TATA or CCAAT element in the expected positions, but multiple putative binding sites for transcriptional factors, such as Sp1, AP-1, AP-2, AP-3, EGR-1, EGR-2, NF-kappaB and HIP1, were detected. We found the existence of a tandem CTT trinucleotide repeat within the 5'-untranslated region (UTR) of the gene, and its polymorphism between C57BL/6J and Mus spretus. Using this polymorphism, the Cchra1 was mapped to the region of chromosome 1 where the synteny to human chromosome 1q was conserved.
K Yamazaki; T Oki; I Tanaka
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Gene     Volume:  214     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  1998 Jul 
Date Detail:
Created Date:  1998-08-31     Completed Date:  1998-08-31     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  199-204     Citation Subset:  IM    
Tsukuba Research Laboratories, Eisai Co., Ltd., 5-1-3, Tokodai, Tsukuba, Ibaraki 300-2635, Japan.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Binding Sites / genetics
Calcium Channels / chemistry,  classification,  genetics*
Chromosome Mapping
Cloning, Molecular
DNA Primers / genetics
Mice, Inbred C57BL
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Genetic
Promoter Regions, Genetic
Species Specificity
Transcription Factors / metabolism
Trinucleotide Repeats
Reg. No./Substance:
0/Calcium Channels; 0/DNA Primers; 0/Transcription Factors

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