| Clonidine and PGE2 have different effects on Na+ and water transport in rat and rabbit CCD. | |
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MedLine Citation:
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PMID: 1858893 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We examined the effects of prostaglandin E2 (PGE2) and the adrenergic agonist clonidine on osmotic water permeability (Pf), lumen-to-bath 22Na+ flux (Jl----b), and transepithelial voltage (VT) in isolated perfused cortical collecting ducts (CCD) from rats and rabbits. Although PGE2 inhibited arginine vasopressin (AVP)-dependent Jl----b and Pf in CCDs from deoxycorticosterone (DOC)-treated and untreated rabbits, 0.1-10 microM PGE2 had no inhibitory effect on any of these transport parameters in CCDs from DOC-treated rats in presence of AVP. On the other hand, clonidine (1 microM in bathing solution) reversibly inhibited AVP-dependent Pf, Jl----b, and VT in the rat CCD by 30-40%, and 0.3 microM yohimbine, a specific alpha 2-adrenoceptor antagonist, reversed these effects. However, we were unable to demonstrate any inhibitory effect of 1-10 microM clonidine on Pf, Jl----b, or VT in the rabbit CCD using a variety of protocols. These results are consistent with the pattern of inhibition of AVP-dependent adenosine 3',5'-cyclic monophosphate (cAMP) production in the rat and rabbit CCD in that PGE2 inhibits both transport and cAMP production in the rabbit but not the rat CCD, and clonidine inhibits both transport and cAMP production in the rat but not the rabbit CCD [D. Chabardès, C. Brick-Ghannam, M. Montégut, and S. Siaume-Perez, Am. J. Physiol. 255 (Renal Fluid Electrolyte Physiol. 24): F43-F48, 1988]. |
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Authors:
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L Chen; M C Reif; J A Schafer |
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Publication Detail:
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Type: Comparative Study; In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 261 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1991 Jul |
Date Detail:
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Created Date: 1991-08-23 Completed Date: 1991-08-23 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: F126-36 Citation Subset: IM |
Affiliation:
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Department of Physiology, University of Alabama, Birmingham 35294. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport / drug effects Clonidine / pharmacology* Desoxycorticosterone / pharmacology Dinoprostone / pharmacology* Electrophysiology Kidney Tubules, Collecting / metabolism, physiology Permeability Rabbits / metabolism* Rats / metabolism* Sodium / pharmacokinetics* Water / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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1-P50-DK-39258/DK/NIDDK NIH HHS; 5-RO1-DK-25519/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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363-24-6/Dinoprostone; 4205-90-7/Clonidine; 64-85-7/Desoxycorticosterone; 7440-23-5/Sodium; 7732-18-5/Water |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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