| Clones of interstitial cells from bovine aortic valve exhibit different calcifying potential when exposed to endotoxin and phosphate. | |
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MedLine Citation:
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PMID: 18832754 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Our purpose was to study in vitro whether phenotypically-distinct interstitial cell clones from bovine aortic valve (BVIC) possess different calcifying potential in response to endotoxin (lipopolysaccharide [LPS]) and phosphate (Pi). METHODS AND RESULTS: Among various clones of BVIC obtained by limited dilution technique we selected 4 clones displaying different growth patterns and immunophenotypes. Uncloned and cloned cells were treated with combinations of LPS (100 ng/mL) and Pi (2.4 mmol/L). Uncloned BVIC showed increased alkaline phosphatase activity (ALP) after treatment with LPS, which resulted in calcification after addition of Pi. Among BVIC clones, only Clone 1 (fibroblast-like phenotype) showed a relevant increase in ALP after LPS treatment in parallel with prevention of smooth muscle (SM) alpha-actin accumulation. No effect was observed in clonal cells harboring a more stable SM cell-like profile (Clone 4). None of the isolated clones calcified but mineralization was induced in the presence of LPS plus Pi when Clone 1 was cocultured with Clone 4 or after seeding on type I collagen sponges. CONCLUSIONS: Endotoxin and phosphate can act as valve calcification promoters by targeting specific fibroblast-like interstitial valve cells that possess a unique procalcific potential. |
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Authors:
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Marcello Rattazzi; Laura Iop; Elisabetta Faggin; Elisa Bertacco; Giacomo Zoppellaro; Ilenia Baesso; Massimo Puato; Gianluca Torregrossa; Gian Paolo Fadini; Carlo Agostini; Gino Gerosa; Saverio Sartore; Paolo Pauletto |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-10-02 |
Journal Detail:
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Title: Arteriosclerosis, thrombosis, and vascular biology Volume: 28 ISSN: 1524-4636 ISO Abbreviation: Arterioscler. Thromb. Vasc. Biol. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-11-21 Completed Date: 2009-01-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9505803 Medline TA: Arterioscler Thromb Vasc Biol Country: United States |
Other Details:
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Languages: eng Pagination: 2165-72 Citation Subset: IM |
Affiliation:
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Dipartimento di Medicina Clinica e Sperimentale, Università degli Studi di Padova, Treviso, Italy. marcello.rattazzi@unipd.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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metabolism Animals Aortic Valve / drug effects*, metabolism, pathology* Calcinosis / etiology*, metabolism, pathology Calcium / metabolism Cattle Clone Cells Coculture Techniques Fibroblasts / drug effects, metabolism, pathology Heart Valve Diseases / etiology*, metabolism, pathology Lipopolysaccharides / toxicity* Myocytes, Smooth Muscle / drug effects, metabolism, pathology Phenotype Phosphates / toxicity* |
| Chemical | |
Reg. No./Substance:
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0/Lipopolysaccharides; 0/Phosphates; 7440-70-2/Calcium; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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