Document Detail


Cloned mouse mammary tumor virus DNA exhibits glucocorticoid-dependent expression in simian virus 40-transformed mink cells.
MedLine Citation:
PMID:  6296429     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mink lung epithelial cells were transfected with two cloned mouse mammary tumor virus (MMTV) DNAs, a 9-kilobase clone derived from an unintegrated exogenous viral genome and a 14-kilobase clone containing an integrated endogenous provirus along with cellular flanking sequences. Mink lung cells were chosen because they do not contain endogenous MMTV sequences. On the basis of our observation that simian virus 40 DNA efficiently transforms these cells, we isolated cell clones containing MMTV DNA by using transformation with simian virus 40 DNA as a selective marker in cotransfection experiments. Levels of the 9-kilobase MMTV mRNA representing the entire viral genome and of the spliced 4.4-kilobase mRNA which codes for the viral envelope proteins were glucocorticoid dependent in transformed cells. Expression of low levels of Pr77gag, the precursor of the group-specific viral core proteins, and of gPr73env, the precursor of the viral envelope proteins, was also hormone dependent. We conclude that these cloned MMTV DNAs contain all the information necessary for the synthesis of normal viral RNAs and proteins. These findings also provide further evidence that the DNA sequences involved in the hormone responsiveness of MMTV expression are contained within the viral genome.
Authors:
D Owen; H Diggelmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of virology     Volume:  45     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1983 Jan 
Date Detail:
Created Date:  1983-03-11     Completed Date:  1983-03-11     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  148-54     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Transformation, Viral
Cloning, Molecular
DNA, Viral / genetics
Dexamethasone / pharmacology*
Gene Expression Regulation / drug effects*
Mammary Tumor Virus, Mouse / genetics*
Mink
RNA, Messenger / biosynthesis
RNA, Viral / biosynthesis
Simian virus 40 / physiology
Transfection*
Viral Core Proteins
Viral Envelope Proteins
Viral Proteins / biosynthesis
Chemical
Reg. No./Substance:
0/DNA, Viral; 0/RNA, Messenger; 0/RNA, Viral; 0/Viral Core Proteins; 0/Viral Envelope Proteins; 0/Viral Proteins; 50-02-2/Dexamethasone
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