Document Detail


Clonality analysis of hematopoiesis and thrombopoietin levels in patients with essential thrombocythemia.
MedLine Citation:
PMID:  9669688     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Essential thrombocythemia (ET) is a myeloproliferative disorder, characterized by sustained thrombocytosis. Diagnosis requires the elimination of all known causes of thrombocytosis. ET is believed to be a clonal disorder, and we investigated the frequency of a clonal hematopoiesis in this disease with the aim of using this as a positive diagnostic criterion. However, a non-random inactivation pattern can be encountered in normal females which mimics clonal hematopoiesis. In addition, the percentage of normal females with skewed lyonization seems higher using techniques based on the difference in DNA methylation, compared to G6PD enzyme polymorphism. Recently, new techniques based on transcript analysis have been developed. We report here the results of clonality studies of hematopoiesis in 53 ET patients using two different techniques based on DNA and RNA polymorphisms, and T-lymphocytes as a control tissue of lyonization. The majority of ET patients showed monoclonal hematopoiesis in the presence of polyclonality of T-lymphocytes. Because all ET patients did not show the same clonal pattern of hematopoiesis, we searched for inappropriate secretion of thrombopoietin (TPO) in patients with polyclonal disease. This assay was performed in 48 patients, of whom 9 showed polyclonal hematopoiesis and 27 monoclonal hematopoiesis. We found no difference in TPO levels between ET patients and normal controls, nor between patients with polyclonal hematopoiesis and those with monoclonal hematopoiesis. Our results confirm the high frequency of monoclonal hematopoiesis in ET, the usefulness of RNA markers, and the possibility of using T-lymphocytes as a control tissue for X-chromosome inactivation patterns. On the other hand, TPO levels are not decreased even in ET patients with high platelet counts, suggesting an increased production or decreased clearance of TPO in this disease.
Authors:
N El-Kassar; G Hetet; J Brière; B Grandchamp
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Publication Detail:
Type:  Clinical Trial; Journal Article    
Journal Detail:
Title:  Leukemia & lymphoma     Volume:  30     ISSN:  1042-8194     ISO Abbreviation:  Leuk. Lymphoma     Publication Date:  1998 Jun 
Date Detail:
Created Date:  1998-10-09     Completed Date:  1998-10-09     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9007422     Medline TA:  Leuk Lymphoma     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  181-8     Citation Subset:  IM    
Affiliation:
INSERM U409, Association Claude Bernard, and Service d'hématologie clinique, Faculté de Medéecine Bichat, Paris, France. U409@bichat.inserm.fr
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Aged, 80 and over
Cell Division / physiology
Cell Lineage
Female
Hematopoiesis / physiology*
Humans
Megakaryocytes / pathology*
Middle Aged
Thrombocytopenia / metabolism,  physiopathology*
Thrombopoietin / metabolism*
Chemical
Reg. No./Substance:
9014-42-0/Thrombopoietin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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