Document Detail

Clonal variation in the Spodoptera frugiperda IPLB-SF21-AE insect cell population.
MedLine Citation:
PMID:  7764939     Owner:  NLM     Status:  MEDLINE    
Clones have been isolated from the heterogeneous Spodoptera frugiperda IPLB-SF21-AE insect cell population. Five of these clones, in addition to the parent cell line and the SF9 cell line (another clonal isolate of the parent cell line), have been compared in regards to morphology, growth, budded virus synthesis, and recombinant protein synthesis. No significant differences in cell morphology were found among these cell lines. There was, however, a significant difference in the average cell size, with diameters ranging from 9.30 +/- 0.184 to 11.11 +/- 0.22 microns and from 9.17 +/- 0.05 to 11.25 +/- 0.24 microns for cells growing in Excell 401 serum-free medium in spinner flask cultures and in TNM-FH medium supplemented with 10% FBS in tissue flask cultures, respectively. While no significant differences in the growth rates were found in TNM-FH medium containing 10% calf serum, significant differences were found in Excell 401 serum-free medium, with population doubling times ranging from 38.5 +/- 6.6 to 64.5 +/- 6.4 h in spinner flask studies. Significant differences in expression levels of Escherichia coli beta-galactosidase (beta-gal) were also found in both 12-well plates and spinner flasks. In the 12-well plate studies, the peak levels of beta-galactosidase obtained by these cell lines ranged from 0.332 +/- 0.091 to 0.805 +/- 0.117 mg/10(6) cells and from 0.580 +/- 0.130 to 1.458 +/- 0.132 mg/10(6) cells in Excell 401 and Hyclone Hy-Q serum-free media, respectively. In the spinner flask studies, peak expression levels ranged from 0.128 +/- 0.053 to 0.573 +/- 0.215 mg/10(6) cells in Excell 401 serum-free medium.(ABSTRACT TRUNCATED AT 250 WORDS)
M K Pasumarthy; D W Murhammer
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Biotechnology progress     Volume:  10     ISSN:  8756-7938     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:    1994 May-Jun
Date Detail:
Created Date:  1994-08-03     Completed Date:  1994-08-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  314-9     Citation Subset:  B    
Department of Chemical and Biochemical Engineering, University of Iowa, Iowa City 52242.
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MeSH Terms
Cell Division / physiology
Cell Size / physiology
Clone Cells / microbiology,  physiology*,  ultrastructure
Moths / cytology*,  microbiology
Nucleopolyhedrovirus / growth & development*
Recombinant Proteins / biosynthesis
Reg. No./Substance:
0/Recombinant Proteins

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