| Clonal spread of both oxyimino-cephalosporin- and cefoxitin-resistant Klebsiella pneumoniae isolates co-producing SHV-2a and DHA-1 beta-lactamase at a burns intensive care unit. | |
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MedLine Citation:
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PMID: 17095195 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Over a 1-month period, a total of 16 ceftriaxone- and cefoxitin-resistant Klebsiella pneumoniae isolates were isolated from 15 patients hospitalised at a burns intensive care unit (ICU). These isolates showed negative results for extended-spectrum beta-lactamase (ESBL) by the Vitek system and were highly resistant to ceftazidime, aztreonam and cefoxitin (minimum inhibitory concentrations > or =128 microg/mL). The bla(SHV-2a) and bla(DHA-1) genes were detected by polymerase chain reaction and sequence analysis. Pulsed-field gel electrophoresis profiles of the isolates were identical. AmpC disk tests for AmpC enzymes as well as double-disk tests and Clinical and Laboratory Standards Institute (CLSI) confirmatory disk tests for ESBLs yielded positive results for all the isolates. However, only three isolates (18.8%) were shown to produce ESBL by CLSI confirmatory tests using broth microdilution. We report the first outbreak of colonisations and infections due to K. pneumoniae isolates co-producing an SHV-2a ESBL and a DHA-1 AmpC beta-lactamase in a Korean hospital, which were suggested to represent a single clonal spread at a burns ICU. In addition, this report presents problems associated with ESBL detection using broth microdilution in isolates that co-produce an ESBL and an AmpC beta-lactamase. |
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Authors:
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Wonkeun Song; Kyu Man Lee; Han-Sung Kim; Jae-Seok Kim; Jonghyun Kim; Seok Hoon Jeong; Kyoung Ho Roh |
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Publication Detail:
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Type: Journal Article Date: 2006-11-13 |
Journal Detail:
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Title: International journal of antimicrobial agents Volume: 28 ISSN: 0924-8579 ISO Abbreviation: Int. J. Antimicrob. Agents Publication Date: 2006 Dec |
Date Detail:
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Created Date: 2006-11-27 Completed Date: 2007-02-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9111860 Medline TA: Int J Antimicrob Agents Country: Netherlands |
Other Details:
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Languages: eng Pagination: 520-4 Citation Subset: IM |
Affiliation:
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Department of Laboratory Medicine, Hallym University College of Medicine, Kangnam Sacred Heart Hospital, 948-1 Daelim-dong, Youngdeungpo-gu, Seoul 150-950, South Korea. swonkeun@hallym.or.kr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Bacterial Agents
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pharmacology Burn Units* Cefoxitin / pharmacology Cephalosporin Resistance* Cephalosporins / pharmacology Disease Outbreaks* Humans Klebsiella Infections / epidemiology*, microbiology Klebsiella pneumoniae / drug effects, enzymology, genetics* Korea / epidemiology Microbial Sensitivity Tests Polymerase Chain Reaction beta-Lactamases / biosynthesis*, genetics |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Cephalosporins; 35607-66-0/Cefoxitin; EC 3.5.2.-/beta-lactamase SHV-2a; EC 3.5.2.6/beta-Lactamases |
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