Document Detail

Clonal heterogeneity in mycosis fungoides and its relationship to clinical course.
MedLine Citation:
PMID:  12384439     Owner:  NLM     Status:  MEDLINE    
Mycosis fungoides (MF) is a cutaneous T-cell lymphoma characterized by multifocal disease and protracted clinical course. The few studies that have assessed T-cell receptor (TCR) gene rearrangements (GRs) present at different anatomic sites in MF have generally reported a common clone. We used a previously validated 4-color polymerase chain reaction (PCR) assay to assess the size and V-family usage of TCR-gamma GRs in 102 concurrent and/or sequential morphologically involved biopsy specimens (91 skin and 11 lymph nodes) from 39 MF patients. This assay detected TCR-gamma clonal GRs in 89 samples (87%) from 36 patients (92%). In 24 patients (77%), an identical clonal GR was present in at least 2 skin samples. However, in one third of these patients, additional different clonal GRs were also noted. Four patients (13%) had clonal GRs that were distinct in different skin samples. In 3 patients (10%), no GR was detected in any sample. In a comparison of lymph node and skin samples, 8 patients had the identical clonal GRs at both sites, 2 patients had different clonal GRs, and 1 patient had no GR identified at either site. Independent of clinical stage, patients who had the same GR detected in multiple concurrent biopsy specimens at the time of diagnosis were more likely to have progressive disease than those who had different GRs (P =.04). Four-color TCR-gamma PCR analysis can uncover multiple distinct clonal GRs in different samples consistent with multiclonal or oligoclonal disease in a significant proportion of MF patients. Demonstration of identical clonal GRs in multiple biopsy specimens at the time of diagnosis may provide prognostic information related to disease progression.
Francisco Vega; Rajyalakshmi Luthra; L Jeffrey Medeiros; Valerie Dunmire; Sang-Joon Lee; Madeleine Duvic; Dan Jones
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Blood     Volume:  100     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2002-10-17     Completed Date:  2002-12-10     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3369-73     Citation Subset:  AIM; IM    
Division of Pathology and Laboratory Medicine, University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA.
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MeSH Terms
Clone Cells / pathology
Disease Progression
Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor*
Lymph Nodes / pathology
Middle Aged
Mycosis Fungoides / genetics,  pathology*
Neoplasm Proteins / genetics
Neoplastic Stem Cells / chemistry,  pathology*
Polymerase Chain Reaction / methods*
Receptors, Antigen, T-Cell, gamma-delta / genetics
Skin / pathology
Grant Support
Reg. No./Substance:
0/Neoplasm Proteins; 0/Receptors, Antigen, T-Cell, gamma-delta

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