| Clonal growth of murine pre-B colony-forming cells and their targeted infection by a retroviral vector: dependence on interleukin-7. | |
| | |
MedLine Citation:
|
PMID: 2407299 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The cDNA for interleukin-7 (IL-7) was recently isolated from a stromal cell line derived from a long-term B-lymphoid culture. We report that purified recombinant murine IL-7 can promote the clonal growth in semi-solid culture of a subpopulation of cells expressing the B220 surface antigen from normal murine bone marrow. These colony-forming cells (CFC-Pre-B) give rise to colonies of 20 to 1,000 cells after 7 days in culture. Morphologic examination of cells within the colonies showed a characteristic lymphoid morphology, and histochemical examination demonstrated an absence of markers associated with granulocyte, macrophage, eosinophil, or megakaryocyte differentiation, as well as an absence of hemoglobinization (indicative or erythroid differentiation). IL-7 was found to specifically enhance the infection of CFC-Pre-B but not CFU-GM when the cytokine was present during a 48-hour co-cultivation period between irradiated, retrovirus-producing psi 2 clones and normal mouse bone marrow cells. In contrast, IL-3 enhanced the infection of CFU-GM but not CFC-Pre-B. Thymidine suiciding studies suggest that this targeted infection is due to specific induction of cycling of CFC-Pre-B by IL-7 and CFU-GM by IL-3. These data demonstrate that IL-7 can target retroviral infection into a specific subpopulation of early B-lymphoid cells (CFC-Pre-B), and that IL-7 cannot directly promote the in vitro clonal growth of myeloid committed progenitor cells (ie, CFU-GM). |
| | |
Authors:
|
D E Williams; A E Namen; D Y Mochizuki; R W Overell |
Related Documents
:
|
3089979 - Differences of host immune response and of sensitivity to chemotherapeutic agents betwe... 1709369 - Purification and partial characterization of a human hematopoietic precursor population. 2936409 - Abnormal in vitro differentiation of peripheral blood clonogenic b cells in common acut... 8822919 - Hematologic effects of flt3 ligand in vivo in mice. 2085699 - Prolonged suppression of myeloid progenitor cell numbers after stopping interferon trea... 12839789 - Development of reverse transcription (rt)-pcr and real-time rt-pcr assays for rapid det... 23598979 - Cd44 integrates signaling in normal stem cell, cancer stem cell and (pre)metastatic nic... 2462609 - Both rat and mouse t cell receptors specific for the encephalitogenic determinant of my... 15155529 - Msx2 and necdin combined activities are required for smooth muscle differentiation in m... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Blood Volume: 75 ISSN: 0006-4971 ISO Abbreviation: Blood Publication Date: 1990 Mar |
Date Detail:
|
Created Date: 1990-04-05 Completed Date: 1990-04-05 Revised Date: 2006-04-21 |
Medline Journal Info:
|
Nlm Unique ID: 7603509 Medline TA: Blood Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1132-8 Citation Subset: AIM; IM |
Affiliation:
|
Department of Experimental Hematology, Immunex Corporation, Seattle, WA 98101. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antigens, Differentiation, B-Lymphocyte / analysis B-Lymphocytes / cytology* Bone Marrow Cells* Cell Cycle Clone Cells Colony-Forming Units Assay Colony-Stimulating Factors / pharmacology Genetic Vectors Granulocyte-Macrophage Colony-Stimulating Factor Growth Substances / pharmacology Hematopoiesis Hematopoietic Stem Cells / cytology* Interleukin-7 / pharmacology* Mice Mice, Inbred C57BL Retroviridae / genetics, growth & development |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, Differentiation, B-Lymphocyte; 0/Colony-Stimulating Factors; 0/Growth Substances; 0/Interleukin-7; 83869-56-1/Granulocyte-Macrophage Colony-Stimulating Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Open-wedge testicular biopsy in childhood acute lymphoblastic leukemia after two years of maintenanc...
Next Document: Chronic myeloid leukemia may be associated with several bcr-abl transcripts including the acute lymp...