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Clonal composition of human multipotent mesenchymal stromal cells.
MedLine Citation:
PMID:  22728032     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Multipotent mesenchymal stromal cells (MMSCs) are a heterogeneous population consisting of cells with a distinct proliferative potential. The aim of this study was to define clonal composition in MMSCs and trace the dynamics of individual clones in MMSCs subpopulations with different proliferative potentials during the process of cultivation. The investigation was performed at single-cell level, using genetically marked cells. Specifically, human bone marrow MMSCs were infected with a lentiviral vector bearing marker gene. Integration site analysis was performed for clones at each passage by ligation-mediated PCR and Southern blot hybridization. Sibling connections between clones and clonal composition of MMSCs culture at each passage were revealed. The MMSCs population contained multiple, different, mainly small clones. It was found that large long-living clones with a high, but limited proliferative potential could be detected rarely in MMSCs population. These data suggest that the human MMSCs population does not fit the "stem cell" criteria; however MMSCs may contain a subpopulation of large clones with a high proliferative potential.
Authors:
Alexey E Bigildeev; Oxana A Zhironkina; Irina N Shipounova; Natalia V Sats; Svetlana Y Kotyashova; Nina I Drize
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-19
Journal Detail:
Title:  Experimental hematology     Volume:  -     ISSN:  1873-2399     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0402313     Medline TA:  Exp Hematol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc. All rights reserved.
Affiliation:
FSBI Hematological Research Center, Ministry of Health and Social Development, Russia Noviy Zikovsky pr, 4, Moscow, 125167, Russia.
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