| Cln3 blocks IME1 transcription and the Ime1-Ume6 interaction to cause the sporulation incompetence in a sake yeast, Kyokai no. 7. | |
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MedLine Citation:
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PMID: 20541107 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Industrial yeasts, including a sake yeast Kyokai no. 7 (K7), are generally unable to sporulate. In K7 (Saccharomyces cerevisiae) cells, IME1 transcription was not induced under sporulation conditions, and K7 cells partially restored sporulation ability when transformed with a multicopy plasmid bearing IME1. However, the mechanisms of sporulation incompetence in industrial yeasts are poorly understood. We demonstrated that the deletion of the G1 cyclin CLN3, a key activator of the cell cycle, allows K7 cells to induce IME1 transcription and sporulate under sporulation conditions. In K7 cells, CLN3 mRNA and protein were not down-regulated despite sporulation conditions. Moreover, using a two-hybrid assay, we found that Ime1-Ume6 interaction was promoted in Cln3-deficient K7 cells. Thus, Cln3 is involved in the mechanism underlying sporulation incompetence by inhibiting IME1 transcription and the Ime1-Ume6 interaction. Based on these findings, we hypothesize that the absence of transmission of nutrient starvation signals to CLN3 leads to sporulation incompetence in K7 cells. |
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Authors:
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Nobushige Nakazawa; Kimio Abe; Yasuhiro Koshika; Kimio Iwano |
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Publication Detail:
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Type: Journal Article Date: 2010-02-08 |
Journal Detail:
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Title: Journal of bioscience and bioengineering Volume: 110 ISSN: 1347-4421 ISO Abbreviation: J. Biosci. Bioeng. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-06-14 Completed Date: 2010-08-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100888800 Medline TA: J Biosci Bioeng Country: Japan |
Other Details:
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Languages: eng Pagination: 1-7 Citation Subset: IM |
Copyright Information:
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2010. Published by Elsevier B.V. |
Affiliation:
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Department of Biotechnology, Faculty of Bioresource Science, Akita Prefectural University, 241-438 Kaidoubata-Nishi, Shimoshinjyo-Nakano, Akita-shi, Akita Prefecture 010-0195, Japan. nnakazawa@akita-pu.ac.jp |
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| MeSH Terms | |
Descriptor/Qualifier:
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Cyclins
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metabolism* Gene Expression Regulation, Fungal Industrial Microbiology Meiosis / genetics Mutation / genetics Nuclear Proteins / metabolism* Protein Binding Repressor Proteins / metabolism Saccharomyces cerevisiae* / cytology, genetics, metabolism Saccharomyces cerevisiae Proteins / metabolism* Signal Transduction / genetics Spores, Fungal / physiology Transcription Factors / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/CLN3 protein, S cerevisiae; 0/Cyclins; 0/IME1 protein, S cerevisiae; 0/Nuclear Proteins; 0/Repressor Proteins; 0/Saccharomyces cerevisiae Proteins; 0/Transcription Factors; 0/UME6 protein, S cerevisiae |
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