Document Detail

Clitocybins, novel isoindolinone free radical scavengers, from mushroom Clitocybe aurantiaca inhibit apoptotic cell death and cellular senescence.
MedLine Citation:
PMID:  19801829     Owner:  NLM     Status:  MEDLINE    
High concentration of intracellular reactive oxygen species (ROS) plays a role in damaging biological systems. We isolated clitocybin A from the culture broth of Clitocybe aurantiaca and then clitocybin B and C derivatives were synthesized from clitocybin A. IMR-90 lung fibroblast cells were pre-treated or post-treated with clitocybin A, B and C to the addition of 100 muM H(2)O(2). These compounds inhibited the level of intracellular reactive oxygen species (ROS) and H(2)O(2)-induced cell death as judged by hypodiploid cell formation. The inhibitory effect of clitocybins on H(2)O(2)-induced cell death was comparable to that with N-acetylcysteine (NAC), a well-known ROS scavenger. The inhibition of H(2)O(2)-induced cell death by clitocybins was mediated by the reduction of caspase 3 and 9 activation, cytochrome c release from mitochondria and the degradation of IkappaB-alpha and IkappaB-beta, which could be resulted in the prevention of cellular senescence. It suggests that clitocybins are novel compounds scavenging ROS and protect cells from apoptosis and cellular senescence.
Eun-Yi Moon; Jin-Mi Oh; Young-Hee Kim; In-Ja Ryoo; Ick-Dong Yoo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  32     ISSN:  1347-5215     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-10-05     Completed Date:  2010-01-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1689-94     Citation Subset:  IM    
Department of Bioscience and Biotechnology, Sejong University, 98 Kunja-Dong Kwangjin-Gu, Seoul 143-747, Republic of Korea.
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MeSH Terms
Acetylcysteine / pharmacology
Apoptosis / drug effects*
Caspase 3 / metabolism
Caspase 9 / metabolism
Cell Aging / drug effects*
Cell Line
Cytochromes c / metabolism
Free Radical Scavengers / chemical synthesis,  pharmacology*
Fungal Proteins / chemical synthesis,  pharmacology*
Hydrogen Peroxide
I-kappa B Proteins / metabolism
Isoindoles / chemical synthesis,  chemistry,  isolation & purification,  pharmacology*
Mitochondria / metabolism
Reg. No./Substance:
0/Free Radical Scavengers; 0/Fungal Proteins; 0/I kappa B beta protein; 0/I-kappa B Proteins; 0/Isoindoles; 0/clitocybin A; 0/clitocybin B; 0/clitocybin C; 139874-52-5/NF-kappaB inhibitor alpha; 616-91-1/Acetylcysteine; 7722-84-1/Hydrogen Peroxide; 9007-43-6/Cytochromes c; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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